1. Recombinant Proteins
  2. Cytokines and Growth Factors
  3. IGF family
  4. IGFBP-5
  5. IGFBP-5 Protein, Cynomolgus (HEK293, His)

IGFBP-5 Protein, Cynomolgus (HEK293, His)

Cat. No.: HY-P77394
COA Handling Instructions

IGFBP-5 protein modulates IGFs, extending their life span and regulating their growth effects. It inhibits or stimulates IGFs, showing its dual function. By altering IGFs' interaction with cell receptors, IGFBP-5 fine-tunes growth-associated signaling pathways, governing cellular responses. This interplay reveals IGFBP-5's multifaceted role in cellular growth and proliferation. IGFBP-5 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived IGFBP-5 protein, expressed by HEK293 , with C-His labeled tag. The total length of IGFBP-5 Protein, Cynomolgus (HEK293, His) is 252 a.a., with molecular weight of ~35 kDa.

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  • Biological Activity

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Description

IGFBP-5 protein modulates IGFs, extending their life span and regulating their growth effects. It inhibits or stimulates IGFs, showing its dual function. By altering IGFs' interaction with cell receptors, IGFBP-5 fine-tunes growth-associated signaling pathways, governing cellular responses. This interplay reveals IGFBP-5's multifaceted role in cellular growth and proliferation. IGFBP-5 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived IGFBP-5 protein, expressed by HEK293 , with C-His labeled tag. The total length of IGFBP-5 Protein, Cynomolgus (HEK293, His) is 252 a.a., with molecular weight of ~35 kDa.

Background

IGFBP-5 protein plays a pivotal role in modulating the activity of insulin-like growth factors (IGFs) by extending their half-life. Within cell culture, IGFBP-5 exhibits a dual regulatory function, demonstrating the capacity to either inhibit or stimulate the growth-promoting effects of IGFs. This dynamic influence underscores the intricacy of IGFBP-5's impact on cellular processes. Notably, IGFBP-5 achieves these regulatory effects by modifying the interaction between IGFs and their cell surface receptors, thereby finely regulating the signaling pathways associated with cellular growth and development. The nuanced interplay between IGFBP-5 and IGFs highlights the sophisticated control mechanisms that govern cellular responses to growth factors, shedding light on the multifaceted role of IGFBP-5 in orchestrating cellular growth and proliferation.

Biological Activity

Measured by its ability to inhibit the biological activity of IGF-I on MCF 7 human breast cancer cells. The ED50 for this effect is 1.063 µg/mL in the presence of 14 ng/mL rhIGF-I, corresponding to a specific activity is 940.734 U/mg.

Species

Cynomolgus

Source

HEK293

Tag

C-His

Accession

G7PLE3 (L21-E272)

Gene ID

101926781  [NCBI]

Molecular Construction
N-term
IGFBP-5 (L21-E272)
Accession # G7PLE3
His
C-term
Synonyms
Insulin-like growth factor-binding protein 5; IBP-5; IGFBP-5; IBP5
AA Sequence

LGSFVHCEPCDEKALSMCPPSPLGCELVKEPGCGCCMTCALAEGQSCGVYTERCAQGLRCLPRQDEEKPLHALLHGRGVCLNEKSYREQVKIERDSREHEEPTTSEMAEETYSPKIFRPKHTRISELKAEAVKKDRRKKLTQSKFVGGAENTAHPRVISAPEMRQESEQGPCRRHMEASLQELKASPRMVPRAVYLPNCDRKGFYKRKQCKPSRGRKRGICWCVDKYGMKLPGMEYVDGDFQCHTFDSSNVE

Molecular Weight

Approximately 35 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

IGFBP-5 Protein, Cynomolgus (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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IGFBP-5 Protein, Cynomolgus (HEK293, His)
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