1. Recombinant Proteins
  2. Cytokines and Growth Factors Receptor Proteins
  3. Interleukin & Receptors Cytokine Receptors
  4. IL-13 Receptor
  5. IL-13R alpha 2 Protein, Canine (HEK293, His)

IL-13R alpha 2 Protein, Canine (HEK293, His)

Cat. No.: HY-P76990
COA Handling Instructions

IL-13R alpha 2 Protein, functioning as a monomer, exhibits a strong binding affinity for interleukin-13 (IL-13). IL-13R alpha 2 Protein, Canine (HEK293, His) is the recombinant canine-derived IL-13R alpha 2 protein, expressed by HEK293, with C-His labeled tag. The total length of IL-13R alpha 2 Protein, Canine (HEK293, His) is 317 a.a., with molecular weight of 39-46 kDa.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

IL-13R alpha 2 Protein, functioning as a monomer, exhibits a strong binding affinity for interleukin-13 (IL-13). IL-13R alpha 2 Protein, Canine (HEK293, His) is the recombinant canine-derived IL-13R alpha 2 protein, expressed by HEK293, with C-His labeled tag. The total length of IL-13R alpha 2 Protein, Canine (HEK293, His) is 317 a.a., with molecular weight of 39-46 kDa.

Background

IL-13R alpha 2 Protein

Biological Activity

Measured by its ability to inhibit IL13-dependent proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.01287 μg/mL, corresponding to a specific activity is 7.770×104 U/mg.

Species

Canine

Source

HEK293

Tag

C-His

Accession

Q95LF0 (S22-T338)

Gene ID

403622  [NCBI]

Molecular Construction
N-term
IL-13Rα2 (S22-T338)
Accession # Q95LF0
His
C-term
Synonyms
Interleukin-13 receptor subunit alpha-1; IL-13RA1; NR4; CD213a1
AA Sequence

SMLSNAEIKVNPPQDFEIVDPGYLGYLSLQWQPPLFPDNFKECTIEYELKYRNIDSENWKTIITKNLHYKDGFDLNKGIEAKINTLLPAQCTNGSEVRSSWAETTYWTSPQGNRETKIQDMDCVYYNWQYLVCSWKPGMGVHFDTNYQLFYWYEGLDHSAECTDYIKVNGKNMGCRFPYLESSDYKDFYICVNGSSESQPIRPSYFIFQLQNIVKPMPPDYLSLTVKNSEEINLKWNMPKGPIPAKCFIYEIEFTEDGTTWVTTTVENEIQITRTSNESQKLCFLVRSKVNIYCSDDGIWSEWSDEQCWKGDIWKET

Molecular Weight

Approximately 38-50 kDa due to glycosylation

Purity

Greater than 95% as determined by reducing SDS-PAGE

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

IL-13R alpha 2 Protein, Canine (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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IL-13R alpha 2 Protein, Canine (HEK293, His)
Cat. No.:
HY-P76990
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