IL-1R9/IL1RAPL2 Protein, Human (HEK293, His)
Based on 1 Customer Validation
Interleukin-1 (IL-1) is a receptor that mediates gene expression for the synthesis of proteins associated with inflammation. IL1RAPL2 is associated with non-syndromic X-linked mental retardation. IL1RAPL2 is specifically expressed in the nervous system and is a candidate gene for central nervous system diseases. IL-1R9/IL1RAPL2 Protein, Human (HEK293, His) is the recombinant human-derived IL-1R9/IL1RAPL2 protein, expressed by HEK293 , with C-His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
Description
Interleukin-1 (IL-1) is a receptor that mediates gene expression for the synthesis of proteins associated with inflammation. IL1RAPL2 is associated with non-syndromic X-linked mental retardation. IL1RAPL2 is specifically expressed in the nervous system and is a candidate gene for central nervous system diseases. IL-1R9/IL1RAPL2 Protein, Human (HEK293, His) is the recombinant human-derived IL-1R9/IL1RAPL2 protein, expressed by HEK293 , with C-His labeled tag.
Background
Interleukin-1 (IL-1) is a receptor that mediates gene expression for the synthesis of proteins associated with inflammation. The IL1RAPL2 protein is a member of the interleukin-1 receptor family and is similar to interleukin-1 receptor helper protein-like 1 (IL1RAPL1). IL-1 plays a key role in the pathogenesis of polymyositis and dermatomyositis. IL1RAPL2 is associated with non-syndromic X-linked mental retardation. IL1RAPL2 is specifically expressed in the nervous system and is a candidate gene for central nervous system diseases[1][2][3][4].
Verified Bioactivity
Immobilized Human IL-1R9 at 0.5 μg/mL (100 μL/well) can bind Human PTPRD. The ED50 for this effect is 136.4 ng/mL.
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
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Bioactivity - ELISA
Bioactivity - ELISA
Technical Parameters
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Species Human
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Source HEK293
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Tag C-6*His
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Accession
Q9NP60 (T17-E356)
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Molecular Construction
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N-term
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IL-1R9 (T17-E356)
Accession # Q9NP60 -
6*His
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C-term
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Protein Length
Extracellular Domain
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Synonyms
IL1RAPL2; IL-1 Receptor Accessory Protein-Like 2; Interleukin 1 Receptor Accessory Protein Like 2; IL1RAPL-2-Related Protein; IL1RAPL-2; IL-1R-9; TIGIRR-1; Interleukin 1 Receptor 9; IL-1R9; Interleukin-1 Receptor 9; IL1R9; IL-1-RAPL-2; Three Immunoglobuli
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AA Sequence
TNLKMVSKRNSVDGCIDWSVDLKTYMALAGEPVRVKCALFYSYIRTNYSTAQSTGLRLMWYKNKGDLEEPIIFSEVRMSKEEDSIWFHSAEAQDSGFYTCVLRNSTYCMKVSMSLTVAENESGLCYNSRIRYLEKSEVTKRKEISCPDMDDFKKSDQEPDVVWYKECKPKMWRSIIIQKGNALLIQEVQEEDGGNYTCELKYEGKLVRRTTELKVTALLTDKPPKPLFPMENQPSVIDVQLGKPLNIPCKAFFGFSGESGPMIYWMKGEKFIEELAGHIREGEIRLLKEHLGEKEVELALIFDSVVEADLANYTCHVENRNGRKHASVLLRKKDLIYKIE
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Predicted Molecular Mass
39.1 kDa
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Molecular Weight
Approximately 50-55 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (264 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Grundtman C, et al. Immunolocalization of interleukin-1 receptors in the sarcolemma and nuclei of skeletal muscle in patients with idiopathic inflammatory myopathies. Arthritis Rheum. 2007 Feb;56(2):674-87. [Content Brief]
[2]. Sana TR, et al. Computational identification, cloning, and characterization of IL-1R9, a novel interleukin-1 receptor-like gene encoded over an unusually large interval of human chromosome Xq22.2-q22.3. Genomics. 2000 Oct 15;69(2):252-62. [Content Brief]
[3]. Ferrante MI, et al. IL1RAPL2 maps to Xq22 and is specifically expressed in the central nervous system. Gene. 2001 Sep 19;275(2):217-21. [Content Brief]
[4]. Jin H, et al. Two novel members of the interleukin-1 receptor gene family, one deleted in Xp22.1-Xp21.3 mental retardation. Eur J Hum Genet. 2000 Feb;8(2):87-94. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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