IL-1R9/IL1RAPL2 Protein, Human (HEK293, His)

Interleukin-1 (IL-1) is a receptor that mediates gene expression for the synthesis of proteins associated with inflammation. IL1RAPL2 is associated with non-syndromic X-linked mental retardation. IL1RAPL2 is specifically expressed in the nervous system and is a candidate gene for central nervous system diseases. IL-1R9/IL1RAPL2 Protein, Human (HEK293, His) is the recombinant human-derived IL-1R9/IL1RAPL2 protein, expressed by HEK293 , with C-His labeled tag.

Interleukin-1 (IL-1) is a receptor that mediates gene expression for the synthesis of proteins associated with inflammation. The IL1RAPL2 protein is a member of the interleukin-1 receptor family and is similar to interleukin-1 receptor helper protein-like 1 (IL1RAPL1). IL-1 plays a key role in the pathogenesis of polymyositis and dermatomyositis. IL1RAPL2 is associated with non-syndromic X-linked mental retardation. IL1RAPL2 is specifically expressed in the nervous system and is a candidate gene for central nervous system diseases^[1][2][3][4].

Immobilized Human IL-1R9 at 0.5 μg/mL (100 μL/well) can bind Human PTPRD. The ED₅₀ for this effect is 136.4 ng/mL.

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  Immobilized Human IL-1R9 at 0.5 μg/mL (100 μL/well) can bind Human PTPRD. The ED₅₀ for this effect is 136.4 ng/mL.

Human

HEK293

C-6*His

Q9NP60 (T17-E356)

26280  [NCBI]

Partial

TNLKMVSKRNSVDGCIDWSVDLKTYMALAGEPVRVKCALFYSYIRTNYSTAQSTGLRLMWYKNKGDLEEPIIFSEVRMSKEEDSIWFHSAEAQDSGFYTCVLRNSTYCMKVSMSLTVAENESGLCYNSRIRYLEKSEVTKRKEISCPDMDDFKKSDQEPDVVWYKECKPKMWRSIIIQKGNALLIQEVQEEDGGNYTCELKYEGKLVRRTTELKVTALLTDKPPKPLFPMENQPSVIDVQLGKPLNIPCKAFFGFSGESGPMIYWMKGEKFIEELAGHIREGEIRLLKEHLGEKEVELALIFDSVVEADLANYTCHVENRNGRKHASVLLRKKDLIYKIE

Approximately 48-55 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

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  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Grundtman C, et al. Immunolocalization of interleukin-1 receptors in the sarcolemma and nuclei of skeletal muscle in patients with idiopathic inflammatory myopathies. Arthritis Rheum. 2007 Feb;56(2):674-87.  [Content Brief]

  [2]. Sana TR, et al. Computational identification, cloning, and characterization of IL-1R9, a novel interleukin-1 receptor-like gene encoded over an unusually large interval of human chromosome Xq22.2-q22.3. Genomics. 2000 Oct 15;69(2):252-62.  [Content Brief]

  [3]. Ferrante MI, et al. IL1RAPL2 maps to Xq22 and is specifically expressed in the central nervous system. Gene. 2001 Sep 19;275(2):217-21.  [Content Brief]

  [4]. Jin H, et al. Two novel members of the interleukin-1 receptor gene family, one deleted in Xp22.1-Xp21.3 mental retardation. Eur J Hum Genet. 2000 Feb;8(2):87-94.  [Content Brief]