1. Recombinant Proteins
  2. Cytokines and Growth Factors
  3. Interleukin & Receptors
  4. IL-2
  5. IL-2 Protein, Rat (145aa, HEK293, C-His)

IL-2 Protein, Rat (145aa, HEK293, C-His)

Cat. No.: HY-P700649
COA Handling Instructions

IL-2 Protein, produced by activated T-cells, NK cells, and helper T-cells, is crucial for immune response and tolerance. It binds to receptor complexes, activating downstream signaling pathways. IL-2 Protein acts as a growth factor, enhances NK-cell activity, promotes B-cell proliferation, and regulates regulatory T-cells. It also participates in differentiating effector T-cell subsets and memory CD8-positive T-cells. IL-2 Protein, Rat (145aa, HEK293, C-His) is the recombinant rat-derived IL-2 protein, expressed by HEK293 , with C-10*His labeled tag. The total length of IL-2 Protein, Rat (145aa, HEK293, C-His) is 145 a.a., with molecular weight of ~18 kDa.

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

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Description

IL-2 Protein, produced by activated T-cells, NK cells, and helper T-cells, is crucial for immune response and tolerance. It binds to receptor complexes, activating downstream signaling pathways. IL-2 Protein acts as a growth factor, enhances NK-cell activity, promotes B-cell proliferation, and regulates regulatory T-cells. It also participates in differentiating effector T-cell subsets and memory CD8-positive T-cells. IL-2 Protein, Rat (145aa, HEK293, C-His) is the recombinant rat-derived IL-2 protein, expressed by HEK293 , with C-10*His labeled tag. The total length of IL-2 Protein, Rat (145aa, HEK293, C-His) is 145 a.a., with molecular weight of ~18 kDa.

Background

IL-2, a cytokine primarily produced by activated CD4-positive helper T-cells, and to a lesser extent by activated CD8-positive T-cells and natural killer (NK) cells, plays pivotal roles in immune response and tolerance. It binds to a receptor complex composed of either the high-affinity trimeric IL-2R (IL2RA/CD25, IL2RB/CD122, and IL2RG/CD132) or the low-affinity dimeric IL-2R (IL2RB and IL2RG). The interaction triggers oligomerization and conformational changes in the IL-2R subunits, initiating downstream signaling that begins with the phosphorylation of JAK1 and JAK3. Consequently, JAK1 and JAK3 phosphorylate the receptor, creating a docking site for the phosphorylation of substrates, including STAT5, activating pathways such as STAT, phosphoinositide-3-kinase/PI3K, and mitogen-activated protein kinase/MAPK. IL-2 functions as a T-cell growth factor, enhances NK-cell cytolytic activity, and fosters robust proliferation of activated B-cells, leading to increased immunoglobulin production. Crucially, IL-2 plays a pivotal role in regulating the adaptive immune system by governing the survival and proliferation of regulatory T-cells, crucial for immune tolerance maintenance. Furthermore, it participates in the differentiation and homeostasis of effector T-cell subsets, including Th1, Th2, Th17, as well as memory CD8-positive T-cells.

Biological Activity

Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.1884 ng/mL, corresponding to a specific activity is 5.307×106 units/mg.

  • Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.1884 ng/mL, corresponding to a specific activity is 5.307×106 units/mg.
Species

Rat

Source

HEK293

Tag

C-10*His

Accession

P17108 (A11-Q155)

Gene ID
Molecular Construction
N-term
IL-2 (A11-Q155)
Accession # P17108
10*His
C-term
Synonyms
Interleukin-2; IL-2; T-cell growth factor; TCGF; Aldesleukin; IL2
AA Sequence

ALTLVLLVNSAPTSSPAKETQQHLEQLLLDLQVLLRGIDNYKNLKLPMMLTFKFYLPKQATELKHLQCLENELGALQRVLDLTQSKSFHLEDAGNFISNIRVTVVKLKGSENKFECQFDDEPATVVEFLRRWIAICQSIISTMTQ

Molecular Weight

Approximately 18 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

IL-2 Protein, Rat (145aa, HEK293, C-His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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IL-2 Protein, Rat (145aa, HEK293, C-His)
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