2. Recombinant Proteins
  3. Cytokines and Growth Factors
  4. Interleukin & Receptors
  5. IL-34
  6. IL-34 Protein, Mouse (HEK293, His, solution)

IL-34 Protein, Mouse (HEK293, His, solution) is the recombinant mouse-derived IL-34, expressed by HEK293 , with His tag.

For research use only. We do not sell to patients.

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Description

IL-34 Protein, Mouse (HEK293, His, solution) is the recombinant mouse-derived IL-34, expressed by HEK293 , with His tag.
Biological Activity

Immobilized Recombinant Rat CSF1R / MCSF Receptor / CD115 Protein (Fc Tag) at 2 μg/ml (100 μl/well) can bind Recombinant Mouse IL-34 Protein (His Tag), the ED50 is 2.649 ng/mL.

Species

Mouse
Source

HEK293
Tag

C-His
Accession

Q8R1R4-1 (N21-P235)
Gene ID

76527
Molecular Construction
N-term
IL-34 (N21-P235)
Accession # Q8R1R4-1
His
C-term
Protein Length

Full Length of Isoform-1 Mature Protein
Synonyms
Interleukin-34; IL-34; C16orf77
AA Sequence

NENLEIWTLTQDKECDLTGYLRGKLQYKNRLQYMKHYFPINYRIAVPYEGVLRVANITRLQKAHVSERELRYLWVLVSLNATESVMDVLLEGHPSWKYLQEVQTLLENVQRSLMDVEIGPHVEAVLSLLSTPGLSLKLVRPKALLDNCFRVMELLYCSCCKQSPILKWQDCELPRLHPHSPGSLMQCTATNVYPLSRQTPTSLPGSPSSSHGSLP

Predicted Molecular Mass
26 kDa
Molecular Weight

Approximately 35-40 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
Glycosylation
Yes
Purity

≥ 90%, as determined by reducing SDS-PAGE.
Appearance

Solution
Formulation

Supplied as a 0.22 μm filtered solution of PBS, 10% glycerol, pH 7.4.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Reconstitution

N/A.
Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping

Shipping with dry ice.
Documentation
SDS (251 KB)

COA (220 KB)

Handling Instructions (2659 KB)

IL-34 Protein, Mouse (HEK293, His, solution) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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