1. Recombinant Proteins
  2. Cytokines and Growth Factors
  3. Interleukin & Receptors
  4. IL-36RA
  5. IL-36RN Protein, Mouse

IL-36RN Protein, Mouse

Cat. No.: HY-P77007
COA Handling Instructions

The IL-36RN protein demonstrates cytokine activity, interleukin-1 receptor antagonist activity, and interleukin-1 receptor binding activity. It negatively regulates interleukin-6 production and is predicted to be located in the cytoplasm and extracellular region. IL-36RN is active in the extracellular space and associated with pustular psoriasis 14, indicating its relevance in skin pathology. It is expressed in the adult stomach (RPKM 9.0) and lung (RPKM 1.0), suggesting its significance in these tissues. IL-36RN Protein, Mouse is the recombinant mouse-derived IL-36RN protein, expressed by E. coli, with tag free. The total length of IL-36RN Protein, Mouse is 154 a.a., with molecular weight of ~17 kDa.

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Description

The IL-36RN protein demonstrates cytokine activity, interleukin-1 receptor antagonist activity, and interleukin-1 receptor binding activity. It negatively regulates interleukin-6 production and is predicted to be located in the cytoplasm and extracellular region. IL-36RN is active in the extracellular space and associated with pustular psoriasis 14, indicating its relevance in skin pathology. It is expressed in the adult stomach (RPKM 9.0) and lung (RPKM 1.0), suggesting its significance in these tissues. IL-36RN Protein, Mouse is the recombinant mouse-derived IL-36RN protein, expressed by E. coli, with tag free. The total length of IL-36RN Protein, Mouse is 154 a.a., with molecular weight of ~17 kDa.

Background

IL-36RN protein is predicted to possess several important functional attributes, including cytokine activity, interleukin-1 receptor antagonist activity, and interleukin-1 receptor binding activity. It plays a role upstream of or within the negative regulation of interleukin-6 production. The protein is predicted to be located in both the cytoplasm and extracellular region, suggesting its potential involvement in diverse cellular processes. Furthermore, IL-36RN is anticipated to be active in the extracellular space. In humans, this gene is associated with pustular psoriasis 14, indicating its relevance in skin pathology. Notably, IL-36RN shares orthology with the human interleukin 36 receptor antagonist (IL36RN). Expression analysis reveals biased expression in the adult stomach (RPKM 9.0) and lung (RPKM 1.0), underscoring its potential significance in these tissues.

Biological Activity

Measured by its ability to inhibit IL-36 beta induced IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.8070 μg/mL, corresponding to a specific activity is 1239.1574 U/mg, in the presence of 15 ng/mL of Recombinant Mouse IL-36 beta.

  • Measured by its ability to inhibit IL-36 beta induced IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells. The ED50for this effect is 0.8070 μg/mL, corresponding to a specific activity is 1239.1574 U/mg, in the presence of 15ng/mL of Recombinant Mouse IL-36 beta.
Species

Mouse

Source

E. coli

Tag

Tag Free

Accession

NP_062324.2 (V3-D156)

Gene ID

54450  [NCBI]

Molecular Construction
N-term
IL-36RN (V3-D156)
Accession # NP_062324.2
C-term
Synonyms
IL-36RA; Interleukin-36 Receptor Antagonist Protein; IL-1RP3; IL-1F5; IL-1L1; FIL1D; IL1HY1
AA Sequence

VLSGALCFRMKDSALKVLYLHNNQLLAGGLHAEKVIKGEEISVVPNRALDASLSPVILGVQGGSQCLSCGTEKGPILKLEPVNIMELYLGAKESKSFTFYRRDMGLTSSFESAAYPGWFLCTSPEADQPVRLTQIPEDPAWDAPITDFYFQQCD

Molecular Weight

Approximately 17 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

IL-36RN Protein, Mouse Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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IL-36RN Protein, Mouse
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