IL-5 Protein, Pig (His)
IL-5 Protein, expressed by T-lymphocytes and NK cells, is vital for eosinophil survival, differentiation, and chemotaxis. It also affects B-cells, inducing immunoglobulin production and growth. IL-5 Protein activates kinases LYN, SYK, and JAK2 through IL5RA and CSF2RB subunits, signaling via RAS-MAPK and JAK-STAT5 pathways. It exists as a disulfide-linked homodimer. IL-5 Protein, Pig (His) is the recombinant pig-derived IL-5 protein, expressed by E. coli , with N-His labeled tag.
- Species: Pig
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
IL-5 Protein, expressed by T-lymphocytes and NK cells, is vital for eosinophil survival, differentiation, and chemotaxis. It also affects B-cells, inducing immunoglobulin production and growth. IL-5 Protein activates kinases LYN, SYK, and JAK2 through IL5RA and CSF2RB subunits, signaling via RAS-MAPK and JAK-STAT5 pathways. It exists as a disulfide-linked homodimer. IL-5 Protein, Pig (His) is the recombinant pig-derived IL-5 protein, expressed by E. coli , with N-His labeled tag.
IL-5 Protein is a homodimeric cytokine predominantly expressed by T-lymphocytes and NK cells, playing a crucial role in the survival, differentiation, and chemotaxis of eosinophils. It also acts on activated and resting B-cells, inducing immunoglobulin production, growth, and differentiation. Mechanistically, IL-5 Protein exerts its biological effects through a receptor composed of IL5RA subunit and the cytokine receptor common subunit beta/CSF2RB, leading to the activation of various kinases such as LYN, SYK, and JAK2. These activated kinases propagate signals through the RAS-MAPK and JAK-STAT5 pathways. IL-5 Protein exists as a disulfide-linked homodimer and interacts with IL5RA and CSF2RB subunits.
Technical Parameters
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Species Pig
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Source E. coli
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Tag N-6*His
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Accession
Q9MYM5 (V22-S134)
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Molecular Construction
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N-term
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6*His
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IL-5 (V22-S134)
Accession # Q9MYM5 -
C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
IL5; T-Cell Replacing Factor; Interleukin 5; EDF; Eosinophil Differentiation Factor; Colony-Stimulating Factor, Eosinophil; Interleukin-5; B-Cell Differentiation Factor I; IL-5; Interleukin 5 (Colony-Stimulating Factor, Eosinophil); TRF; B Cell Differenti
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AA Sequence
VENTMNRLVAETLTLLSIHRTLLIGDGNLMISTPVHTNHQLCIEEVFQGIDTLKNQTARGDAVEKLFQNLSLIKEYIDRQKKNCGGERWRVTQFLDYLQVFLGVINTEWTMES
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Predicted Molecular Mass
17 kDa
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Molecular Weight
Approximately 14-18 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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