IL-7 Protein, Mouse (HEK293, His)

IL-7, a hematopoietic cytokine, regulates lymphocyte population and maintains lymphoid homeostasis. IL-7 binds to its receptor complex IL7RA and CSF2RG, activating kinases like JAK1 or JAK3 depending on the cell type. This triggers downstream signaling pathways, including PI3K/Akt/mTOR or JAK-STAT5, leading to various cellular responses. IL-7's interaction with IL7R and CSF2RG is crucial for its diverse effects. IL-7 Protein, Mouse (HEK293, His) is the recombinant mouse-derived IL-7 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of IL-7 Protein, Mouse (HEK293, His) is 129 a.a., with molecular weight of 22-28 kDa.

IL-7, a hematopoietic cytokine, plays a pivotal role in the development, expansion, and survival of both naive and memory T-cells and B-cells, thereby regulating the population of mature lymphocytes and maintaining lymphoid homeostasis. Its biological effects are mediated through a receptor complex consisting of the IL7RA subunit and the cytokine receptor common subunit gamma/CSF2RG. Upon binding to the receptor, IL-7 activates various kinases, such as JAK1 or JAK3, depending on the cell type, leading to the propagation of signals through downstream signaling pathways, including PI3K/Akt/mTOR or JAK-STAT5. This interaction with its receptor components IL7R and CSF2RG is central to the diverse cellular responses elicited by IL-7.

1.Mouse IL-7RA-Fc can bind Mouse IL-7-His with an affinity constant of 18.9 nM.
2.Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBL).The ED₅₀ for this effect is 60-1000 pg/mL.

Mouse

HEK293

C-6*His

P10168 (E26-I154)

16196  [NCBI]

ECHIKDKEGKAYESVLMISIDELDKMTGTDSNCPNNEPNFFRKHVCDDTKEAAFLNRAARKLKQFLKMNISEEFNVHLLTVSQGTQTLVNCTSKEEKNVKEQKKNDACFLKRLLREIKTCWNKILKGSI

22-28 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.