NA/Neuraminidase Protein, Influenza B (EPI1868374, sf9, His)
NA/Neuraminidase Protein, Influenza B (EPI1868374, sf9, His) is the recombinant virus-derived NA/Neuraminidase protein, expressed by Sf9 insect cells, with C-His tag.
- Species: Virus
- Source: Sf9 insect cells
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
NA/Neuraminidase Protein, Influenza B (EPI1868374, sf9, His) is the recombinant virus-derived NA/Neuraminidase protein, expressed by Sf9 insect cells, with C-His tag.
Background
The NA/Neuraminidase protein plays a crucial role in viral and cellular glycoconjugates by catalyzing the removal of terminal sialic acid residues. During virus budding, it facilitates the release of progeny virus by cleaving off terminal sialic acids on the glycosylated HA, preventing self-aggregation and ensuring efficient spread from cell to cell. Acting as a receptor-destroying enzyme, NA removes terminal sialic acid from cellular receptors, allowing the virus to spread through the circulation by further removing sialic acids from the cell surface. This activity is vital for avoiding limitations to one round of replication. Moreover, NA may enhance viral invasion of the upper airways by cleaving sialic acid moieties on airway epithelial cell mucin. Its association with lipid rafts during intracellular transport suggests a role in the budding process, potentially influencing host range restriction on replication and virulence. Notably, the sialidase activity within late endosome/lysosome traffic appears to enhance virus replication, highlighting the multifaceted functions of NA in the viral life cycle.
Technical Parameters
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Species Virus
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Source Sf9 insect cells
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Tag C-His
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Accession
EPI1868374 (Y33-L466)
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Gene ID/
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Synonyms
NA; Neuraminidase; NA/Neuraminidase Protein, Influenza B (B/Austria/1359417/2021)
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AA Sequence
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Predicted Molecular Mass
53.5 kDa
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Molecular Weight
Approximately 60 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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