Integrin alpha IV beta 1 Protein, Human (HEK293, His)
Based on 1 Customer Validation
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Verified Bioactivity
Measured by its binding ability in a functional ELISA. Immobilized Natalizumab at 1 μg/mL (100 μL/well) can bind Human ITGA4&ITGB1 Heterodimer Protein, His Tag&Tag Free with a linear range of 30 -150 ng/mL.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-His
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Accession
P13612-1 (Y34-T977)& P05556-1 (Q21-D728)
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Gene ID3676&3688
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Molecular Construction
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N-term
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ITGA4 (Y34-T977)
Accession # P13612-1 -
His
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C-term
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N-term
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ITGB1 (Q21-D728)
Accession # P05556-1 -
C-term
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Synonyms
ITGA4; Very Late Activation Protein 4 Receptor, Alpha 4 Subunit; Prev. CD49D; Integrin A4-Subunit; CD49d; Integrin A4 Subunit; Alpha 4 Subunit Of VLA-4 Receptor; Alpha-4 Integrin; CD49 Antigen-Like Family Member D; 269C Wild Type; VLA-4 Subunit Alpha; Ant
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Predicted Molecular Mass
111.5 kDa (ITGA4) & 83.7 kDa (ITGB1)
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Molecular Weight
Approximately 60-75 kDa (ITGB1) & 110-140 kDa (ITGA4), based on SDS-PAGE under non reducing (N) conditions, due to the glycosylation.
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Glycosylation
Yes
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Structure/Form
Heterodimer
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
Lyophilized from 0.22 μm filtered solution of 50 mM Tris, 150 mM NaCl, pH 7.5 with 10% trehalose as protectant.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (232 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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