IRS1 Protein, Human (His, Strep)
IRS1 Protein, Human (His, Strep) is the recombinant human-derived IRS1, expressed by E. coli , with Strep, His labeled tag. ,
- Species: Human
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
IRS1 Protein, Human (His, Strep) is the recombinant human-derived IRS1, expressed by E. coli , with Strep, His labeled tag. ,
Background
IRS-1 is a signaling adapter protein that mediates signal transduction from the insulin receptor (INSR) and insulin-like growth factor I receptor (IGF1R). It plays a critical role in development, growth, glucose homeostasis, and lipid metabolism. Upon phosphorylation by the insulin receptor, IRS-1 acts as a signaling scaffold, propagating insulin action by binding to SH2 domain-containing proteins such as the p85 regulatory subunit of PI3K, NCK1, NCK2, GRB2, or SHP2. Recruitment of GRB2 activates the guanine nucleotide exchange factor SOS1, triggering the Ras/Raf/MEK/MAPK signaling cascade (by similar). The PI3K/AKT pathway, activated by IRS-1, mediates most of insulin's metabolic effects, while the Ras/Raf/MEK/MAPK pathway regulates gene expression and, in coordination with PI3K, controls cell growth and differentiation. Additionally, IRS-1 positively regulates the Wnt/β-catenin signaling pathway by suppressing autophagy-mediated degradation of DVL2, thereby promoting cell proliferation.
Technical Parameters
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Species Human
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Source E. coli
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Tag Strep;His
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Accession
P35568 (P4-R267)
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Gene ID3667
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Protein Length
Partial
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Synonyms
IRS1; HIRS-1; Insulin Receptor Substrate 1; IRS-1
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AA Sequence
PPESDGFSDVRKVGYLRKPKSMHKRFFVLRAASEAGGPARLEYYENEKKWRHKSSAPKRSIPLESCFNINKRADSKNKHLVALYTRDEHFAIAADSEAEQDSWYQALLQLHNRAKGHHDGAAALGAGGGGGSCSGSSGLGEAGEDLSYGDVPPGPAFKEVWQVILKPKGLGQTKNLIGIYRLCLTSKTISFVKLNSEAAAVVLQLMNIRRCGHSENFFFIEVGRSAVTGPGEFWMQVDDSVVAQNMHETILEAMRAMSDEFRPR
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Predicted Molecular Mass
32.3 kDa
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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