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Jagged-1/JAG1 Protein, Rat (HEK293, His)

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Based on 1 Customer Validation

Jagged-1/JAG1 protein is a ligand of multiple Notch receptors, mediates Notch signaling, affects cell fate decisions during hematopoiesis and enhances angiogenesis. It functions throughout mammalian cardiovascular development, regulates myoblast differentiation, and participates in the complex Notch signaling network through interactions with NOTCH1, NOTCH2, and NOTCH3. Jagged-1/JAG1 Protein, Rat (HEK293, His) is the recombinant rat-derived Jagged-1/JAG1 protein, expressed by HEK293.

For research use only. We do not sell to patients.
  • Species: Rat
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Jagged-1/JAG1 Protein, Rat (HEK293, His)
  • Experimental Validation Results for Jagged-1/JAG1 Protein, Rat (HEK293, His)
  • Jagged-1/JAG1 Protein, Rat (HEK293, His) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

Jagged-1/JAG1 protein is a ligand of multiple Notch receptors, mediates Notch signaling, affects cell fate decisions during hematopoiesis and enhances angiogenesis. It functions throughout mammalian cardiovascular development, regulates myoblast differentiation, and participates in the complex Notch signaling network through interactions with NOTCH1, NOTCH2, and NOTCH3. Jagged-1/JAG1 Protein, Rat (HEK293, His) is the recombinant rat-derived Jagged-1/JAG1 protein, expressed by HEK293.

Background

Jagged-1 (JAG1) serves as a ligand for multiple Notch receptors, playing a pivotal role in mediating Notch signaling. It is implicated in cell-fate decisions during hematopoiesis and demonstrates the capacity to enhance fibroblast growth factor-induced angiogenesis in vitro. JAG1's involvement spans various stages of mammalian cardiovascular development, highlighting its significance in these processes. Additionally, it exerts an inhibitory effect on myoblast differentiation, suggesting its regulatory role in diverse cellular functions. Through interactions with NOTCH1, NOTCH2, and NOTCH3, JAG1 contributes to the intricate network of Notch signaling, underscoring its multifaceted role in cellular responses and developmental pathways.

Verified Bioactivity

Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 1.264 μg/mL, corresponding to a specific activity is 791.1392 units/mg.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 90%, as determined by reducing SDS-PAGE.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 1.264 μg/mL, corresponding to a specific activity is 791.1392 units/mg.

Technical Parameters

  • Species Rat
  • Source HEK293
  • Tag C-6*His
  • Accession

    Q63722 (S32-D1067, with substitutions GGARN56-60AE and DR63-64TLVRPY)

  • Gene ID
    29146
  • Protein Length

    Partial Extracellular Domain

  • Synonyms

    JAG1; Jagged 1; Prev. JAGL1; Alagille Syndrome; Prev. AGS; Jagged-1 Protein; HJ1; CD339 Antigen; CD339; Jagged1; AHD; CMT2HH; AWS; AGS1; Protein Jagged-1; DCHE; Jagged Canonical Notch Ligand 1; Delta-Like Protein

  • AA Sequence

    SGQFELEILSMQNVNGELQNGNCCAEPGTLVRPYKCTRDECDTYFKVCLKEYQSRVTAGGPCSFGSGSTPVIGGNTFNLKASRGNDRNRIVLPFSFAWPRSYTLLVEAWDSSNDTIQPDSIIEKASHSGMINPSRQWQTLKQNTGIAHFEYQIRVTCDDHYYGFGCNKFCRPRDDFFGHYACDQNGNKTCMEGWMGPECNKAICRQGCSPKHGSCKLPGDCRCQYGWQGLYCDKCIPHPGCVHGTCNEPWQCLCETNWGGQLCDKDLNYCGTHQPCLNRGTCSNTGPDKYQCSCPEGYSGPNCEIAEHACLSDPCHNRGSCKETSSGFECECSPGWTGPTCSTNIDDCSPNNCSHGGTCQDLVNGFKCVCPPQWTGKTCQLDANECEAKPCVNARSCKNLIASYYCDCLPGWMGQNCDININDCLGQCQNDASCRDLVNGYRCICPPGYAGDHCERDIDECASNPCLNGGHCQNEINRFQCLCPTGFSGNLCQLDIDYCEPNPCQNGAQCYNRASDYFCKCPEDYEGKNCSHLKDHCRTTPCEVIDSCTVAMASNDTPEGVRYISSNVCGPHGKCKSESGGKFTCDCNKGFTGTYCHENINDCEGNPCTNGGTCIDGVNSYKCICSDGWEGAHCENNINDCSQNPCHYGGTCRDLVNDFYCDCKNGWKGKTCHSRDSQCDEATCNNGGTCYDEVDTFKCMCPGGWEGTTCNIARNSSCLPNPCHNGGTCVVNGDSFTCVCKEGWEGPICTQNTNDCSPHPCYNSGTCVDGDNWYRCECAPGFAGPDCRININECQSSPCAFGATCVDEINGYQCICPPGHSGAKCHEVSGRSCITMGRVILDGAKWDDDCNTCQCLNGRVACSKVWCGPRPCRLHKGHGECPNGQSCIPVLDDQCFVRPCTGAGECRSSSLQPVKTKCTSDSYYQDNCANITFTFNKEMMSPGLTTEHICSELRNLNILKNVSAEYSIYIACEPSLSANNEIHVAISAEDIRDDGNPVKEITDKIIDLVSKRDGNSSLIAAVAEVRVQRRPLKNRTD

  • Predicted Molecular Mass

    114.2 kDa

  • Molecular Weight

    Approximately 125-130 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in PBS.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 90%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 1.264 μg/mL, corresponding to a specific activity is 791.1392 units/mg.

  • Jagged-1/JAG1 Protein, Rat (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for Jagged-1/JAG1 Protein, Rat (HEK293, His)
  • Experimental Validation Results for Jagged-1/JAG1 Protein, Rat (HEK293, His)
  • Jagged-1/JAG1 Protein, Rat (HEK293, His) Product Image