KLC2 Protein, Human (sf9, His-GST)

The KLC2 protein is a key kinesin-1 complex component that is essential for the generation of microtubule-associated forces and organelle transport. Its light chain facilitates the coupling of cargo to the heavy chain and regulates ATPase activity. KLC2 Protein, Human (sf9, His-GST) is the recombinant human-derived KLC2 protein, expressed by Sf9 insect cells , with C-His, N-GST labeled tag.

KLC2, a crucial component of the kinesin-1 motor complex, is integral to microtubule-associated force generation and plays a vital role in organelle transport. The light chain of KLC2 is implicated in facilitating the coupling of cargo to the heavy chain and modulating its ATPase activity. By interacting with PLEKHM2 and ARL8B, KLC2 orchestrates the recruitment of kinesin-1 to lysosomes, thereby guiding the movement of lysosomes towards microtubule plus ends. The oligomeric complex formed by KLC2 consists of two heavy chains and two light chains. This interaction, particularly through the TPR repeats, highlights KLC2's involvement in regulating the intricate machinery of organelle transport within the cell.

Human

Sf9 insect cells

C-His;N-GST

Q9H0B6-1 (M1-G622)

64837  [NCBI]

Full Length of Isoform-1

MAMMVFPREEKLSQDEIVLGTKAVIQGLETLRGEHRALLAPLVAPEAGEAEPGSQERCILLRRSLEAIELGLGEAQVILALSSHLGAVESEKQKLRAQVRRLVQENQWLREELAGTQQKLQRSEQAVAQLEEEKQHLLFMSQIRKLDEDASPNEEKGDVPKDTLDDLFPNEDEQSPAPSPGGGDVSGQHGGYEIPARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSGHDHPDVATMLNILALVYRDQNKYKEAAHLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGKFHPDVAKQLSNLALLCQNQGKAEEVEYYYRRALEIYATRLGPDDPNVAKTKNNLASCYLKQGKYQDAETLYKEILTRAHEKEFGSVNGDNKPIWMHAEEREESKDKRRDSAPYGEYGSWYKACKVDSPTVNTTLRSLGALYRRQGKLEAAHTLEDCASRNRKQGLDPASQTKVVELLKDGSGRRGDRRSSRDMAGGAGPRSESDLEDVGPTAEWNGDGSGSLRRSGSFGKLRDALRRSSEMLVKKLQGGTPQEPPNPRMKRASSLNFLNKSVEEPTQPGGTGLSDSRTLSSSSMDLSRRSSLVG

≥ 90%, as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.