KLHL21 Protein, Human (sf9, His, StrepⅡ)

KLHL21 acts as a substrate-specific linker in the BCR E3 ubiquitin ligase complex to ensure efficient chromosome alignment and cytokinesis. The BCR(KLHL21) complex coordinates chromosomal passenger complex (CPC) dynamics, directing its transition from chromosomes to the spindle midzone during anaphase. KLHL21 Protein, Human (sf9, His, Strep) is the recombinant human-derived KLHL21 protein, expressed by sf9 insect cells , with N-Strep, N-8*His labeled tag.

KLHL21, serving as the substrate-specific adapter within the BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex, plays a crucial role in ensuring efficient chromosome alignment and cytokinesis. The BCR(KLHL21) E3 ubiquitin ligase complex is instrumental in orchestrating the localization dynamics of the chromosomal passenger complex (CPC) during anaphase, facilitating its transition from chromosomes to the spindle midzone. Furthermore, this complex actively mediates the ubiquitination of AURKB, a key player in the regulation of cell division. It is noteworthy that, despite the ubiquitination of AURKB by the BCR(KLHL21) E3 ubiquitin ligase complex, this may not necessarily lead to its degradation by the proteasome, highlighting the nuanced nature of protein modification processes governed by KLHL21. The intricate interplay within the BCR(KLHL21) complex underscores its pivotal role in the regulation of cell division through precise control of CPC localization and AURKB ubiquitination, thus contributing to the orchestration of essential cellular events.

Human

Sf9 insect cells

N-StrepⅡ;N-8*His

Q9UJP4 (E2-H597)

9903

Partial

ERPAPLAVLPFSDPAHALSLLRGLSQLRAERKFLDVTLEAAGGRDFPAHRAVLAAASPYFRAMFAGQLRESRAERVRLHGVPPDMLQLLLDFSYTGRVAVSGDNAEPLLRAADLLQFPAVKEACGAFLQQQLDLANCLDMQDFAEAFSCSGLASAAQRFILRHVGELGAEQLERLPLARLLRYLRDDGLCVPKEEAAYQLALRWVRADPPRRAAHWPQLLEAVRLPFVRRFYLLAHVEAEPLVARCPPCLRLLREARDFQAARYDRHDRGPCPRMRPRPSTGLAEILVLVGGCDQDCDELVTVDCYNPQTGQWRYLAEFPDHLGGGYSIVALGNDIYVTGGSDGSRLYDCVWRYNSSVNEWAEVAPMLKAREYHSSSVLDGLLYVVAADSTERYDHTTDSWEALQPMTYPMDNCSTTACRGRLYAIGSLAGKETMVMQCYDPDTDLWSLVDCGQLPPWSFAPKTATLNGLMYFVRDDSAEVDVYNPTRNEWDKIPSMNQVHVGGSLAVLGGKLYVSGGYDNTFELSDVVEAYDPETRAWSVVGRLPEPTFWHGSVSIFRQFMPQTFSGGRGFELDSGSDDMDPGRPRPPRDPDELH

69.7 KDa

≥ 90%, as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 50 mM HEPES, pH 7.5, 500 mM NaCl, 10% glycerol, 1 mM DTT.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. /biology-dictionary/nci-h1299-cell.html  [Content Brief]