KLHL42 Protein, Human (sf9, His, StrepⅡ)

KLHL42 serves as a substrate-specific linker in the BCR E3 ubiquitin ligase complex and plays a critical role in mitotic progression and cytokinesis. The BCR(KLHL42) complex actively ubiquitinates and degrades KATNA1, contributing to the regulation of microtubule dynamics during mitosis. KLHL42 Protein, Human (sf9, His, Strep) is the recombinant human-derived KLHL42 protein, expressed by sf9 insect cells , with N-Strep, N-8*His labeled tag.

KLHL42, functioning as the substrate-specific adapter within the BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex, assumes a pivotal role in mitotic progression and cytokinesis. The BCR(KLHL42) E3 ubiquitin ligase complex orchestrates these essential cellular processes by actively mediating the ubiquitination and subsequent degradation of KATNA1, thereby contributing to the regulation of microtubule dynamics throughout mitosis. The involvement of KLHL42 in protein modification, specifically protein ubiquitination, highlights its significance in fine-tuning the cellular events crucial for proper mitotic progression and cytokinesis. The intricate interplay within the BCR(KLHL42) complex underscores its importance in maintaining the delicate balance required for the dynamic processes associated with cell division.

Human

Sf9 insect cells

N-StrepⅡ;N-8*His

Q9P2K6 (S2-T505)

57542

Partial

SAEEMVQIRLEDRCYPVSKRKLIEQSDYFRALYRSGMREALSQEAGGPEVQQLRGLSAPGLRLVLDFINAGGAREGWLLGPRGEKGGGVDEDEEMDEVSLLSELVEAASFLQVTSLLQLLLSQVRLNNCLEMYRLAQVYGLPDLQEACLRFMVVHFHEVLCKPQFHLLGSPPQAPGDVSLKQRLREARMTGTPVLVALGDFLGGPLAPHPYQGEPPSMLRYEEMTERWFPLANNLPPDLVNVRGYGSAILDNYLFIVGGYRITSQEISAAHSYNPSTNEWLQVASMNQKRSNFKLVAVNSKLYAIGGQAVSNVECYNPEQDAWNFVAPLPNPLAEFSACECKGKIYVIGGYTTRDRNMNILQYCPSSDMWTLFETCDVHIRKQQMVSVEETIYIVGGCLHELGPNRRSSQSEDMLTVQSYNTVTRQWLYLKENTSKSGLNLTCALHNDGIYIMSRDVTLSTSLEHRVFLKYNIFSDSWEAFRRFPAFGHNLLVSSLYLPNKAET

≥ 90%, as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.