Klotho beta Protein, Human (968a.a, HEK293, His)
Based on 1 Customer Validation
Klotho beta protein plays a critical role in the transcriptional repression of cholesterol 7-alpha-hydroxylase (CYP7A1), a key enzyme controlling bile acid synthesis. Although its glycosidase activity may be inactive, Klotho beta enhances the binding ability of FGFR1 and FGFR4 to FGF21, promoting their interaction. Klotho beta Protein, Human (968a.a, HEK293, His) is the recombinant human-derived Klotho beta protein, expressed by HEK293, with C-His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
Klotho beta protein plays a critical role in the transcriptional repression of cholesterol 7-alpha-hydroxylase (CYP7A1), a key enzyme controlling bile acid synthesis. Although its glycosidase activity may be inactive, Klotho beta enhances the binding ability of FGFR1 and FGFR4 to FGF21, promoting their interaction. Klotho beta Protein, Human (968a.a, HEK293, His) is the recombinant human-derived Klotho beta protein, expressed by HEK293, with C-His labeled tag.
Background
The Klotho beta protein plays a significant role in transcriptional regulation by contributing to the repression of cholesterol 7-alpha-hydroxylase (CYP7A1), a key enzyme in bile acid synthesis. While likely inactive as a glycosidase, Klotho beta enhances the binding ability of FGFR1 and FGFR4 to FGF21, indicating its involvement in fibroblast growth factor (FGF) signaling. The protein directly interacts with both FGF19 and FGF21, underscoring its association with crucial components of the FGF pathway. These interactions, particularly with FGF21, suggest a potential role in metabolic regulation and highlight Klotho beta's involvement in modulating cellular responses and metabolic pathways (
Technical Parameters
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Species Human
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Source HEK293
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Tag C-His
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Accession
Q86Z14 (M30-L997)
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Gene ID152831
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Molecular Construction
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N-term
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Klotho beta (M30-L997)
Accession # Q86Z14 -
His
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C-term
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Protein Length
Partial Extracellular Domain
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Synonyms
KLB; B-Klotho; Klotho Beta; BKL; Beta-Klotho; Klotho Beta Like; BetaKlotho; KLB Protein; Klotho Beta-Like Protein
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AA Sequence
MSNGGLQRSVILSALILLRAVTGFSGDGRAIWSKNPNFTPVNESQLFLYDTFPKNFFWGIGTGALQVEGSWKKDGKGPSIWDHFIHTHLKNVSSTNGSSDSYIFLEKDLSALDFIGVSFYQFSISWPRLFPDGIVTVANAKGLQYYSTLLDALVLRNIEPIVTLYHWDLPLALQEKYGGWKNDTIIDIFNDYATYCFQMFGDRVKYWITIHNPYLVAWHGYGTGMHAPGEKGNLAAVYTVGHNLIKAHSKVWHNYNTHFRPHQKGWLSITLGSHWIEPNRSENTMDIFKCQQSMVSVLGWFANPIHGDGDYPEGMRKKLFSVLPIFSEAEKHEMRGTADFFAFSFGPNNFKPLNTMAKMGQNVSLNLREALNWIKLEYNNPRILIAENGWFTDSRVKTEDTTAIYMMKNFLSQVLQAIRLDEIRVFGYTAWSLLDGFEWQDAYTIRRGLFYVDFNSKQKERKPKSSAHYYKQIIRENGFSLKESTPDVQGQFPCDFSWGVTESVLKPESVASSPQFSDPHLYVWNATGNRLLHRVEGVRLKTRPAQCTDFVNIKKQLEMLARMKVTHYRFALDWASVLPTGNLSAVNRQALRYYRCVVSEGLKLGISAMVTLYYPTHAHLGLPEPLLHADGWLNPSTAEAFQAYAGLCFQELGDLVKLWITINEPNRLSDIYNRSGNDTYGAAHNLLVAHALAWRLYDRQFRPSQRGAVSLSLHADWAEPANPYADSHWRAAERFLQFEIAWFAEPLFKTGDYPAAMREYIASKHRRGLSSSALPRLTEAERRLLKGTVDFCALNHFTTRFVMHEQLAGSRYDSDRDIQFLQDITRLSSPTRLAVIPWGVRKLLRWVRRNYGDMDIYITASGIDDQALEDDRLRKYYLGKYLQEVLKAYLIDKVRIKGYYAFKLAEEKSKPRFGFFTSDFKAKSSIQFYNKVISSRGFPFENSSSRCSQTQENTECTVCLFLVQKKPL
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Predicted Molecular Mass
112.9 kDa
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Molecular Weight
Approximately 120-130 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from 0.22 μm filtered solution of PBS, pH 7.4 with 10% trehalose as protectant.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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