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L1CAM Protein, Human (HEK293, His, solution)

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Based on 2 publication(s) in Google Scholar

The L1CAM protein is a neural cell adhesion molecule that plays a critical role in cell adhesion dynamics and initiation of tyrosine kinase receptor transmembrane signaling. Its importance spans brain development, affecting processes such as neuronal migration, axonal growth, fasciculations, and synaptogenesis. L1CAM Protein, Human (HEK293, His, solution) is the recombinant human-derived L1CAM protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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609-228-6898
[email protected] [email protected]
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

The L1CAM protein is a neural cell adhesion molecule that plays a critical role in cell adhesion dynamics and initiation of tyrosine kinase receptor transmembrane signaling. Its importance spans brain development, affecting processes such as neuronal migration, axonal growth, fasciculations, and synaptogenesis. L1CAM Protein, Human (HEK293, His, solution) is the recombinant human-derived L1CAM protein, expressed by HEK293 , with C-His labeled tag.

Background

L1CAM, a neural cell adhesion molecule, intricately participates in the modulation of cell adhesion dynamics and the initiation of transmembrane signals at tyrosine kinase receptors. Its significance spans various stages of brain development, where it proves critical in processes such as neuronal migration, axonal growth, fasciculation, and synaptogenesis. In the mature brain, L1CAM continues to play a pivotal role in regulating the dynamics of neuronal structure and function, notably contributing to synaptic plasticity. This multifaceted protein interacts with SHTN1, with the interaction prominently occurring in axonal growth cones, and engages with isoform 2 of BSG, underscoring its involvement in diverse cellular functions.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-His
  • Accession

    P32004-1 (I20-E1120)

  • Gene ID
    3897  [NCBI]
  • Molecular Construction
    • N-term
    • L1CAM (I20-E1120)
      Accession # P32004-1
    • His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    L1CAM; NCAM-L1; Prev. MIC5; CD171; Prev. HSAS1; Antigen Identified By Monoclonal Antibody R1; Prev. HSAS; N-CAM-L1; Prev. MASA; CD171 Antigen; Prev. SPG1; N-CAML1; Prev. S10; HYCX; Neural Cell Adhesion Molecule L1; L1 Cell Adhesion Molecule; CAML1

  • AA Sequence

    IQIPEEYEGHHVMEPPVITEQSPRRLVVFPTDDISLKCEASGKPEVQFRWTRDGVHFKPKEELGVTVYQSPHSGSFTITGNNSNFAQRFQGIYRCFASNKLGTAMSHEIRLMAEGAPKWPKETVKPVEVEEGESVVLPCNPPPSAEPLRIYWMNSKILHIKQDERVTMGQNGNLYFANVLTSDNHSDYICHAHFPGTRTIIQKEPIDLRVKATNSMIDRKPRLLFPTNSSSHLVALQGQPLVLECIAEGFPTPTIKWLRPSGPMPADRVTYQNHNKTLQLLKVGEEDDGEYRCLAENSLGSARHAYYVTVEAAPYWLHKPQSHLYGPGETARLDCQVQGRPQPEVTWRINGIPVEELAKDQKYRIQRGALILSNVQPSDTMVTQCEARNRHGLLLANAYIYVVQLPAKILTADNQTYMAVQGSTAYLLCKAFGAPVPSVQWLDEDGTTVLQDERFFPYANGTLGIRDLQANDTGRYFCLAANDQNNVTIMANLKVKDATQITQGPRSTIEKKGSRVTFTCQASFDPSLQPSITWRGDGRDLQELGDSDKYFIEDGRLVIHSLDYSDQGNYSCVASTELDVVESRAQLLVVGSPGPVPRLVLSDLHLLTQSQVRVSWSPAEDHNAPIEKYDIEFEDKEMAPEKWYSLGKVPGNQTSTTLKLSPYVHYTFRVTAINKYGPGEPSPVSETVVTPEAAPEKNPVDVKGEGNETTNMVITWKPLRWMDWNAPQVQYRVQWRPQGTRGPWQEQIVSDPFLVVSNTSTFVPYEIKVQAVNSQGKGPEPQVTIGYSGEDYPQAIPELEGIEILNSSAVLVKWRPVDLAQVKGHLRGYNVTYWREGSQRKHSKRHIHKDHVVVPANTTSVILSGLRPYSSYHLEVQAFNGRGSGPASEFTFSTPEGVPGHPEALHLECQSNTSLLLRWQPPLSHNGVLTGYVLSYHPLDEGGKGQLSFNLRDPELRTHNLTDLSPHLRYRFQLQATTKEGPGEAIVREGGTMALSGISDFGNISATAGENYSVVSWVPKEGQCNFRFHILFKALGEEKGGASLSPQYVSYNQSSYTQWDLQPDTDYEIHLFKERMFRHQMAVKTNGTGRVRLPPAGFATE

  • Predicted Molecular Mass

    125 kDa

  • Molecular Weight

    Approximately 160-200 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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