LAMP2/CD107b Protein, Human (HEK293, His)

LAMP2 is a key lysosomal glycoprotein that plays crucial roles in lysosomal biogenesis, pH regulation, and autophagy. It directly inhibits the TMEM175 proton channel, optimizing lysosomal acidity for efficient hydrolase activity. LAMP2/CD107b Protein, Human (HEK293, His) is the recombinant human-derived LAMP2/CD107b protein, expressed by HEK293 , with C-6*His labeled tag.

LAMP2, a lysosomal membrane glycoprotein, assumes a pivotal role in lysosome biogenesis, lysosomal pH regulation, and autophagy. It acts as a crucial regulator of lysosomal lumen pH by directly inhibiting the proton channel TMEM175, facilitating optimal lysosomal acidification for effective hydrolase activity. LAMP2 plays a significant role in chaperone-mediated autophagy, participating in the lysosomal degradation of proteins during various stresses and as part of the normal turnover of long-lived proteins. This function involves binding target proteins such as GAPDH, NLRP3, and MLLT11 and directing them for lysosomal degradation. In chaperone-mediated autophagy, LAMP2 operates downstream of chaperones like HSPA8/HSC70, which recognize and bind substrate proteins, mediating their recruitment to lysosomes where LAMP2 facilitates binding. Furthermore, LAMP2 is essential for the fusion of autophagosomes with lysosomes during autophagy, influencing the degradation of autophagosomal contents. Additionally, LAMP2 is required for the efficient presentation of exogenous antigens via MHCII-mediated pathways, as it modulates chaperone-mediated autophagy and decreases the presentation of endogenous antigens by MHCII, highlighting its multifaceted roles in cellular processes.

Human

HEK293

C-6*His

P13473-1 (L29-F375)

3920  [NCBI]

Lumenal Domain

LELNLTDSENATCLYAKWQMNFTVRYETTNKTYKTVTISDHGTVTYNGSICGDDQNGPKIAVQFGPGFSWIANFTKAASTYSIDSVSFSYNTGDNTTFPDAEDKGILTVDELLAIRIPLNDLFRCNSLSTLEKNDVVQHYWDVLVQAFVQNGTVSTNEFLCDKDKTSTVAPTIHTTVPSPTTTPTPKEKPEAGTYSVNNGNDTCLLATMGLQLNITQDKVASVININPNTTHSTGSCRSHTALLRLNSSTIKYLDFVFAVKNENRFYLKEVNISMYLVNGSVFSIANNNLSYWDAPLGSSYMCNKEQTVSVSGAFQINTFDLRVQPFNVTQGKYSTAQECSLDDDTI

Approximately 60-120 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.