Lgr5/GPR49 Protein, Cynomolgus (HEK293, His)
The Lgr5/GPR49 protein is a receptor for R-spondins that enhances the canonical Wnt signaling pathway and marks stem cells in the intestinal epithelium and hair follicles. Lgr5/GPR49 binds R-spondins (RSPO1-4), binds to phosphorylated LRP6 and Frizzled receptors, initiates Wnt signaling and amplifies target gene expression. Lgr5/GPR49 Protein, Cynomolgus (HEK293, His) is the recombinant human-derived Lgr5/GPR49 protein, expressed by HEK293 , with C-His labeled tag.
- Species: Cynomolgus
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
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Biological Activity
Description
The Lgr5/GPR49 protein is a receptor for R-spondins that enhances the canonical Wnt signaling pathway and marks stem cells in the intestinal epithelium and hair follicles. Lgr5/GPR49 binds R-spondins (RSPO1-4), binds to phosphorylated LRP6 and Frizzled receptors, initiates Wnt signaling and amplifies target gene expression. Lgr5/GPR49 Protein, Cynomolgus (HEK293, His) is the recombinant human-derived Lgr5/GPR49 protein, expressed by HEK293 , with C-His labeled tag.
Background
The Lgr5/GPR49 Protein serves as the receptor for R-spondins, functioning to potentiate the canonical Wnt signaling pathway while concurrently serving as a distinctive stem cell marker in the intestinal epithelium and hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3, or RSPO4), Lgr5/GPR49 associates with phosphorylated LRP6 and frizzled receptors activated by extracellular Wnt, initiating the canonical Wnt signaling pathway and amplifying the expression of target genes. In contrast to classical G-protein coupled receptors, Lgr5/GPR49 does not activate heterotrimeric G-proteins to transduce the signal, highlighting its unique regulatory role in Wnt signaling. It plays a crucial role in the development and maintenance of adult intestinal stem cells during postembryonic development and has been identified in a complex with RNF43 and RSPO1. Additionally, Lgr5/GPR49 interacts with other R-spondin ligands, including RSPO2, RSPO3, and RSPO4, emphasizing its diverse participation in cellular signaling pathways.
Technical Parameters
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Species Cynomolgus
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Source HEK293
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Tag C-His
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Accession
G7PI19-1 (S22-G557)
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Gene ID/
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Molecular Construction
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N-term
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LGR5 (S22-G557)
Accession # G7PI19-1 -
His
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C-term
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Protein Length
Partial
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Synonyms
LGR5; G-Protein Coupled Receptor HG38; Prev. GPR49; G-Protein Coupled Receptor 49; Prev. GPR67; G-Protein Coupled Receptor 67; HG38; Leucine-Rich Repeat-Containing G Protein-Coupled Receptor 5, Isoform CRA_b; FEX; Orphan G Protein-Coupled Receptor HG38; L
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AA Sequence
SSPRSGALLRGCPTHCHCEPDGRMLLRVDCSDLGLSELPSNLSVFTSYLDLSMNNISQLLPNPLPSLRFLEELRLAGNALTYIPKGAFTGLYSLKVLMLQNNQLRQVPTEALQNLRSLQSLRLDANHISYVPPSCFSGLHSLRHLWLDDNALTEIPVQAFRSLSALQAMTLALNKIHHIPDYAFGNLSSLVVLHLHNNRIHSLGKKCFDGLHSLETLDLNYNNLDEFPTAIRTLSNLKELGFHSNNIRSIPEKAFVGNPSLITIHFYDNPIQFVGRSAFQHLPELRTLTLNGASQITEFPDLTGTANLESLTLTGAQISSLPQTVCNQLPNLQVLDLSYNLLEDLPSFSVCQKLQKIDLRHNEIYEIKVDTFQQLLSLRSLNLAWNKIAIIHPNAFSTLPSLIKLDLSSNLLSSFPVTGLHGLTHLKLTGNHALQSLISSENFPELKIIEMPYAYQCCAFGVCENAYKISNQWNKGDNSSMDDLHKKDAGMFQVQDERDLEDFLLDFEEDLKALHSVQCSPSPGPFKPCEHLLDG
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Predicted Molecular Mass
61.7 kDa
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Molecular Weight
Approximately 66-70 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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