LIMS1 Protein, Mouse (sf9, His)
LIMS1 is an adapter protein that critically connects β-integrin to the actin cytoskeleton and cell surface receptors. It mediates important cellular processes and competes with LIMS2 by linking to integrin-linked protein kinase 1 (ILK) through its first LIM domain. LIMS1 Protein, Mouse (sf9, His) is the recombinant mouse-derived LIMS1 protein, expressed by sf9 insect cells , with N-10*His labeled tag.
- Species: Mouse
- Source: Sf9 insect cells
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
LIMS1 is an adapter protein that critically connects β-integrin to the actin cytoskeleton and cell surface receptors. It mediates important cellular processes and competes with LIMS2 by linking to integrin-linked protein kinase 1 (ILK) through its first LIM domain. LIMS1 Protein, Mouse (sf9, His) is the recombinant mouse-derived LIMS1 protein, expressed by sf9 insect cells , with N-10*His labeled tag.
Background
LIMS1, functioning as an adapter protein within a cytoplasmic complex, serves as a crucial link between beta-integrins and the actin cytoskeleton, while simultaneously connecting the complex to cell surface receptor tyrosine kinases and growth factor receptors. Its involvement spans the regulation of essential cellular processes, including cell survival, proliferation, and differentiation. Through the first LIM domain, LIMS1 interacts with integrin-linked protein kinase 1 (ILK), engaging in competitive binding with LIMS2. Notably, LIMS1 contributes to the heterotrimeric IPP complex, alongside ILK and either LIMS1 or LIMS2, which plays a pivotal role in cellular signaling. Furthermore, LIMS1 establishes connections with the SH3/SH2 adapter NCK2, facilitating the linkage of the complex to cell surface receptors. Its interaction with TGFB1I1, mediated by the LIM zinc-binding 5 domain, further expands its network of molecular associations.
Technical Parameters
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Species Mouse
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Source Sf9 insect cells
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Tag N-10*His
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Accession
Q99JW4 (A2-K325)
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Molecular Construction
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N-term
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10*His
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LIMS1 (A2-K325)
Accession # Q99JW4 -
C-term
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Protein Length
Full Length
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Synonyms
LIMS1; LIM And Senescent Cell Antigen-Like Domains 1; LIM Zinc Finger Domain Containing 1; Renal Carcinoma Antigen NY-REN-48; Particularly Interesting New Cys-His Protein 1; LIM-Type Zinc Finger Domains 1; PINCH1; Senescent Cell Antigen; PINCH; PINCH-1; L
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AA Sequence
ANALASATCERCKGGFAPAEKIVNSNGELYHEQCFVCAQCFQQFPEGLFYEFEGRKYCEHDFQMLFAPCCHQCGEFIIGRVIKAMNNSWHPECFRCDLCQEVLADIGFVKNAGRHLCRPCHNREKARGLGKYICQKCHAIIDEQPLIFKNDPYHPDHFNCANCGKELTADARELKGELYCLPCHDKMGVPICGACRRPIEGRVVNAMGKQWHVEHFVCAKCEKPFLGHRHYERKGLAYCETHYNQLFGDVCFHCNRVIEGDVVSALNKAWCVSCFACSTCNTKLTLKNKFVEFDMKPVCKKCYEKFPLELKKRLKKLSETLGRK
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Predicted Molecular Mass
39.6 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US;may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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