LIMS1 Protein, Mouse (sf9, His)

LIMS1 is an adapter protein that critically connects β-integrin to the actin cytoskeleton and cell surface receptors. It mediates important cellular processes and competes with LIMS2 by linking to integrin-linked protein kinase 1 (ILK) through its first LIM domain. LIMS1 Protein, Mouse (sf9, His) is the recombinant mouse-derived LIMS1 protein, expressed by sf9 insect cells , with N-10*His labeled tag.

LIMS1, functioning as an adapter protein within a cytoplasmic complex, serves as a crucial link between beta-integrins and the actin cytoskeleton, while simultaneously connecting the complex to cell surface receptor tyrosine kinases and growth factor receptors. Its involvement spans the regulation of essential cellular processes, including cell survival, proliferation, and differentiation. Through the first LIM domain, LIMS1 interacts with integrin-linked protein kinase 1 (ILK), engaging in competitive binding with LIMS2. Notably, LIMS1 contributes to the heterotrimeric IPP complex, alongside ILK and either LIMS1 or LIMS2, which plays a pivotal role in cellular signaling. Furthermore, LIMS1 establishes connections with the SH3/SH2 adapter NCK2, facilitating the linkage of the complex to cell surface receptors. Its interaction with TGFB1I1, mediated by the LIM zinc-binding 5 domain, further expands its network of molecular associations.

Mouse

Sf9 insect cells

N-10*His

Q99JW4 (A2-K325)

110829  [NCBI]

Full Length of Mature Protein

ANALASATCERCKGGFAPAEKIVNSNGELYHEQCFVCAQCFQQFPEGLFYEFEGRKYCEHDFQMLFAPCCHQCGEFIIGRVIKAMNNSWHPECFRCDLCQEVLADIGFVKNAGRHLCRPCHNREKARGLGKYICQKCHAIIDEQPLIFKNDPYHPDHFNCANCGKELTADARELKGELYCLPCHDKMGVPICGACRRPIEGRVVNAMGKQWHVEHFVCAKCEKPFLGHRHYERKGLAYCETHYNQLFGDVCFHCNRVIEGDVVSALNKAWCVSCFACSTCNTKLTLKNKFVEFDMKPVCKKCYEKFPLELKKRLKKLSETLGRK

≥ 90%, as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.