lipoxygenase Protein, Pseudomonas aeruginosa
The lipoxygenase protein catalyzes the oxygen-mediated conversion of linoleic acid to 9HPOD and spontaneously decomposes it into 9HOD. It converts linolenic acid into 13HPOD, which breaks down into 13HOD. lipoxygenase Protein, Pseudomonas aeruginosa is the recombinant lipoxygenase protein, expressed by E. coli , with tag free.
- Species: Others
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
The lipoxygenase protein catalyzes the oxygen-mediated conversion of linoleic acid to 9HPOD and spontaneously decomposes it into 9HOD. It converts linolenic acid into 13HPOD, which breaks down into 13HOD. lipoxygenase Protein, Pseudomonas aeruginosa is the recombinant lipoxygenase protein, expressed by E. coli , with tag free.
Background
In the presence of oxygen, the lipoxygenase protein catalyzes the conversion of linoleate into (9S)-hydroperoxy-10,12-octadecenoate (9HPOD), with subsequent spontaneous decomposition into the corresponding 9-hydroxy-10,12-octadecenoate (9HOD). It also transforms linolenate into its hydroperoxy derivative, 13-hydroperoxy-9,11-octadecenoate (13HPOD), which spontaneously decomposes to the corresponding 13-hydroxy-9,11-octadecenoate (13HOD). Additionally, the enzyme exhibits activity on oleate, albeit to a lesser extent, converting it into (10S)-hydroperoxy-8E-octadecenoate, which undergoes spontaneous decomposition to form the corresponding 10-hydroxy-8E-octadecenoate. Notably, lipoxygenase demonstrates minimal activity on arachidonate. This enzymatic versatility highlights its role in mediating diverse fatty acid oxygenation reactions, contributing to the intricate metabolic pathways associated with lipid signaling and inflammation.
Technical Parameters
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Species Others
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Source E. coli
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Tag Tag Free
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Accession
BAQ41224.1 (M1-I678)
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Gene ID/
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Molecular Construction
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N-term
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lipoxygenase (M1-I678)
Accession # BAQ41224.1 -
C-term
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Protein Length
Partial
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Synonyms
LOX; Lysyl Oxidase Homolog; Lysyl Oxidase; AAT10; Protein-Lysine 6-Oxidase
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AA Sequence
MSGVALSGTALANDSIFFSPLKYLGAEQQRSIDASRSLLDNLIPPSLPQYDNLAGKLARRAVLTSKKLVYVWTENFANVKGVPMARSVPLGELPNVDWLLKTAGVIVELIVNFVASLPASAAAQFERIAAGLSGDLEAARQVHEALLEEAKNDPAAAGSLLLRFTELQTRVIALLTRVGLLVDDILKSASNLVTQGGQGDGLNRFRAVFGTLRLPEVADSFRDDEAFAYWRVAGPNPLLIRRVDALPANFPLGEEQFRRVMGADDSLLEAAASRRLYLLDYAELGKLAPSGAVDKLLTGTGFAYAPIALFALGKDRARLLPVAIQCGQDPVTHPMFVRPAESESDLYWGWQMAKTVVQVAEENYHEMFVHLAQTHLVSEAFCLATQRTLAPSHPLHVLLAPHFEGTLFINEGAARILLPSAGFIDVMFAAPIQDTQATAGGNRLGFDFYRGMLPESLKARNVDDPAALPDYPYRDDGLLVWNAIRQWAADYVAVYYASDGDVTGDVELAAWVGEVIGSGKVAGFRPITGRSQLVEVLTMVIFTASAQHAAVNFPQPSMMTYAPAICAMSAAPAPDSPSGKSEADWLKMMPPTLVALEKVNIYHLLGSVYHGRLGDYRQTGFPYAPVFSDRRVTASGGPLERFQARLKEVEATIRTRNQARRKPYEYLLPSRIPASTNI
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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