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  4. LRG1 Protein, Rat (HEK293, His)

LRG1 Protein, Rat (HEK293, His) is the recombinant rat-derived LRG1 protein, expressed by HEK293, with C-8*His tag.

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Beschreibung

LRG1 Protein, Rat (HEK293, His) is the recombinant rat-derived LRG1 protein, expressed by HEK293, with C-8*His tag.
Background

LRG1 is a member of the leucine-rich repeat sequence (LRR) family of proteins involved in protein-protein interactions, signal transduction, and cell adhesion and development. LRG1 is involved in promoting neovascularization (new blood vessel growth) by causing the switching of transforming growth factor β (TGF-β) signaling in endothelial cells. LRG1 binds to the co-receptor endorphins and promotes signaling through the ALK1-Smad1/5/8 pathway. LRG1 is involved in ovarian cancer progression by activating the FAK/AKT signaling pathway[1][2][3].
Species

Rat
Source

HEK293
Tag

C-8*His
Accession

XP_038939952.1 (K33-L342)
Gene ID

367455
Molecular Construction
N-term
LRG1 (K33-L342)
Accession # XP_038939952.1
8*His
C-term
Protein Length

Partial
Synonyms
rHuLeucine-rich alpha-2-glycoprotein/LRG1, His; Leucine-rich alpha-2-glycoprotein; HMFT1766; LRG; LRG1
AA Sequence

KGSTVSCHGPTEFPSSLPADTVHLSVEFSSLTQLPAAALQGCPGLQELHLSSNRLQELSPGLLAPVPRLRVLDLTRNALRSLPPGLFRSSAALNTLVLRENQLQEASARWLQGLDALGYLDLAENRLRSLPARLLANLGALHTLDLGHNLLESLPEGFLRGPRRLQRLHLEGNRLQRLEAGLLAPQPFLGVLFLNDNQLTAVAADSFRGL

Predicted Molecular Mass
35.6 kDa
Molekulargewicht

Approximately 42-52 kDa,based on SDS-PAGE under reducing conditions,due to the glycosylation.
Reinheit

≥ 95%, as determined by Bis-Tris PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Dokumentation
Handling Instructions (2659 KB)

LRG1 Protein, Rat (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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