LRRFIP1 Protein, Human
Based on 1 Customer Validation
LRRFIP1 is a transcriptional repressor that preferentially binds to GC-rich sequences (5'-AGCCCCCGGCG-3') and may regulate the expression of TNF, EGFR, and PDGFA. It inhibits PDGFA, which controls smooth muscle cell proliferation after arterial injury. LRRFIP1 Protein, Human is the recombinant human-derived LRRFIP1 protein, expressed by E. coli.
- Species: Human
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
LRRFIP1 is a transcriptional repressor that preferentially binds to GC-rich sequences (5'-AGCCCCCGGCG-3') and may regulate the expression of TNF, EGFR, and PDGFA. It inhibits PDGFA, which controls smooth muscle cell proliferation after arterial injury. LRRFIP1 Protein, Human is the recombinant human-derived LRRFIP1 protein, expressed by E. coli.
LRRFIP1, functioning as a transcriptional repressor, exhibits a preference for binding to the GC-rich consensus sequence (5'-AGCCCCCGGCG-3') and is implicated in the potential regulation of TNF, EGFR, and PDGFA expression. This regulatory role extends to the control of smooth muscle cell proliferation following artery injury, achieved through the repression of PDGFA. Furthermore, LRRFIP1 demonstrates the capacity to bind double-stranded RNA. It plays a positive role in Toll-like receptor (TLR) signaling in response to agonists, likely by competitively interacting with the negative FLII regulator for MYD88-binding. Existing as a homodimer, LRRFIP1 may also form higher-order oligomers, and it interacts with FLII and MYD88, competing with FLII for MyD88-binding, even in the absence of lipopolysaccharide (LPS).
Technical Parameters
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Species Human
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Source E. coli
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Tag Tag Free
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Accession
Q32MZ4 (D162-E249)
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Gene ID9208
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Molecular Construction
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N-term
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LRRFIP1 (D162-E249)
Accession # Q32MZ4 -
C-term
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Protein Length
Partial
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Synonyms
LRRFIP1; Leucine-rich repeat flightless-interacting protein 1; LRR FLII-interacting protein 1; GC-binding factor 2; TAR RNA-interacting protein
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Molecular Weight
Approximately 12 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, pH 7.5, 150mM NaCl, 5% glycerol.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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