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  4. CHRM1 Protein, Human (HEK293, Flag)

CHRM1 Protein, Human (HEK293, Flag) is the recombinant human-derived M1R, expressed by HEK293 , with Flag labeled tag.

For research use only. We do not sell to patients.

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Description

CHRM1 Protein, Human (HEK293, Flag) is the recombinant human-derived M1R, expressed by HEK293 , with Flag labeled tag.
Background

M1R is a muscarinic acetylcholine receptor that mediates various cellular responses, primarily through the action of G proteins, including inhibition of adenylate cyclase, breakdown of phosphoinositides, and modulation of potassium channels. Its primary transducing effect is Pi turnover.
Species

Human
Source

HEK293
Tag

Flag
Accession

P11229-1 (M1-A219, S112R, F355-C460)
Gene ID

1128
Protein Length

Partial
Synonyms
Muscarinic acetylcholine receptor M1; CHRM1; Homo sapiens; Human; G-protein coupled receptor; Receptor; Transducer
AA Sequence

FSLVKEKKAARTLSAILLAFILTWTPYNIMVLVSTFCKDCVPETLWELGYWLCYVNSTINPMCYALCNKAFRDTFRLLLLCRWDKRRWRKIPKRPGSVHRTPSRQC

Predicted Molecular Mass
56.8 kDa
Purity

≥ 85%, as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

CHRM1 Protein, Human (HEK293, Flag) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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