MASP2 Protein, Human (His)
Based on 1 Customer Validation
MASP2 Protein, a serum protease, critically activates the complement system by auto-catalytically cleaving and subsequently cleaving C2 and C4. This process leads to the activation of C3 and the formation of C3 convertase, essential for the complement cascade. MASP2 Protein, Human (His) is the recombinant human-derived MASP2 protein, expressed by E. coli , with C-His labeled tag.
- Species: Human
- Source: E. coli
-
Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
MASP2 Protein, a serum protease, critically activates the complement system by auto-catalytically cleaving and subsequently cleaving C2 and C4. This process leads to the activation of C3 and the formation of C3 convertase, essential for the complement cascade. MASP2 Protein, Human (His) is the recombinant human-derived MASP2 protein, expressed by E. coli , with C-His labeled tag.
MASP2 is a serum protease that plays a crucial role in the activation of the complement system through mannose-binding lectin. Once activated by auto-catalytic cleavage, MASP2 cleaves C2 and C4, resulting in their activation and the subsequent formation of C3 convertase, a pivotal step in the complement cascade.
1. Immobilized Human MASP2 at 0.2 μg/mL (100μL/Well). Dose response curve for Anti-MASP2 Antibody with the EC50 of 3.0-5.3 ng/mL determined by ELISA.
2. Immobilized Human MASP2 at 0.5 μg/mL (100μL/Well). Dose response curve for Anti-MASP2 Antibody with the EC50 of 1.2-5.3 ng/mL determined by ELISA.
3. Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-LysThioBenzyl ester (Z-Lys-SBzl), in the presence of 5,5'Dithio-bis. The specific activity is ≥3000 pmol/min/µg.
4. Immobilized Human MASP2, His Tag at 0.5 μg/mL (100 μL/Well). Dose response curve for Narsoplimab, hFc Tag with the EC50 of 1.0-10.0 ng/mL determined by ELISA.
Technical Parameters
-
Species Human
-
Source E. coli
-
Tag C-6*His
-
Accession
O00187-1 (G288-F686)
-
Molecular Construction
-
N-term
-
MASP2 (G288-F686)
Accession # O00187-1 -
6*His
-
C-term
-
-
Protein Length
Partial
-
Synonyms
MASP2; Mannan-Binding Lectin Serine Protease 1 Pseudogene 1; Prev. MASP1P1; Mannose-Binding Lectin Associated Protein 19; Mannan-Binding Lectin Serine Protease 2; MBL-Associated Serine Protease 2; Mannose-Binding Protein-Associated Serine Protease 2; MASP
-
AA Sequence
GWKIHYTSTAQPCPYPMAPPNGHVSPVQAKYILKDSFSIFCETGYELLQGHLPLKSFTAVCQKDGSWDRPMPACSIVDCGPPDDLPSGRVEYITGPGVTTYKAVIQYSCEETFYTMKVNDGKYVCEADGFWTSSKGEKSLPVCEPVCGLSARTTGGRIYGGQKAKPGDFPWQVLILGGTTAAGALLYDNWVLTAAHAVYEQKHDASALDIRMGTLKRLSPHYTQAWSEAVFIHEGYTHDAGFDNDIALIKLNNKVVINSNITPICLPRKEAESFMRTDDIGTASGWGLTQRGFLARNLMYVDIPIVDHQKCTAAYEKPPYPRGSVTANMLCAGLESGGKDSCRGDSGGALVFLDSETERWFVGGIVSWGSMNCGEAGQYGVYTKVINYIPWIENIISDF
-
Predicted Molecular Mass
46.9 kDa
-
Molecular Weight
Approximately 18 kDa & 25-30 kDa, based on SDS-PAGE under reducing conditions.
-
Purity
≥ 95%, as determined by Bis-Tris PAGE.
Product Properties
Solution
1.Supplied as a 0.22 μm filtered solution of 50 mM Tris, 100-200 mM NaCl, pH 9.0.
2.Supplied as a 0.22 μm filtered solution of 20 mM Tris, 200 mM NaCl, pH 9.0.
3.Supplied as a 0.22 μm filtered solution of 20 mM Tris, 200 mM NaCl, pH 9.0, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
-
Data Sheet (238 KB)
-
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
-
Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
