1. Recombinant Proteins
  2. Complement System
  3. MASP-2
  4. MASP2 Protein, Human (His)

MASP2 Protein, Human (His)

Cat. No.: HY-P78005
COA Handling Instructions

MASP2 Protein, a serum protease, critically activates the complement system by auto-catalytically cleaving and subsequently cleaving C2 and C4. This process leads to the activation of C3 and the formation of C3 convertase, essential for the complement cascade. MASP2 Protein, Human (His) is the recombinant human-derived MASP2 protein, expressed by E. coli , with C-His labeled tag. The total length of MASP2 Protein, Human (His) is 399 a.a., with molecular weight of 18 kDa & 25-30 kDa, respectively.

For research use only. We do not sell to patients.

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Size Price Stock Quantity
20 μg $320 In-stock
50 μg $704 In-stock
100 μg   Get quote  

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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

MASP2 Protein, a serum protease, critically activates the complement system by auto-catalytically cleaving and subsequently cleaving C2 and C4. This process leads to the activation of C3 and the formation of C3 convertase, essential for the complement cascade. MASP2 Protein, Human (His) is the recombinant human-derived MASP2 protein, expressed by E. coli , with C-His labeled tag. The total length of MASP2 Protein, Human (His) is 399 a.a., with molecular weight of 18 kDa & 25-30 kDa, respectively.

Background

MASP2 is a serum protease that plays a crucial role in the activation of the complement system through mannose-binding lectin. Once activated by auto-catalytic cleavage, MASP2 cleaves C2 and C4, resulting in their activation and the subsequent formation of C3 convertase, a pivotal step in the complement cascade.

Biological Activity

Immobilized Human MASP2 at 0.2 μg/mL (100μL/Well). Dose response curve for Anti-MASP2 Antibody with the EC50 of 3.0-5.3 ng/mL determined by ELISA.

Species

Human

Source

E. coli

Tag

C-His

Accession

O00187 (G288-F686)

Gene ID
Molecular Construction
N-term
MASP2 (G288-F686)
Accession # O00187
His
C-term
Synonyms
MAP19; MASP-2; MASP1P1; sMap
Molecular Weight

18 kDa&25-30 kDa

Purity

Greater than 95% as determined by Tris-Bis PAGE.

Appearance

Solution.

Formulation

Supplied as a 0.22 μm filtered solution of 50 mM Tris, 100-200 mM NaCl, pH 9.0.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

N/A.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

MASP2 Protein, Human (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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MASP2 Protein, Human (His)
Cat. No.:
HY-P78005
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