MeCP2 Protein, Human (HEK293, His)

The MeCP2 protein is a chromosome binder that specifically binds to methylated DNA independent of flanking sequences. MeCP2 Protein, Human (HEK293, His) is the recombinant human-derived MeCP2 protein, expressed by HEK293 , with C-6*His labeled tag.

The MeCP2 protein functions as a chromosomal entity with a distinctive ability to bind specifically to methylated DNA, targeting individual methyl-CpG pairs independently of flanking DNA sequences. Operating as a mediator of transcriptional repression, MeCP2 engages in interactions with histone deacetylase and the corepressor SIN3A. Moreover, it demonstrates dual binding capabilities, associating with both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, exhibiting a notable preference for 5-methylcytosine (5mC). The versatility of MeCP2 is further emphasized by its interactions with FNBP3, CDKL5, ATRX, NCOR2, TBL1XR1, and TBL1X, each interaction contributing to various cellular processes, including the recruitment of co-repressor complexes and the orchestration of heterochromatin organization. This multifaceted functionality underscores the pivotal role of MeCP2 in epigenetic regulation and the modulation of gene expression.

Human

HEK293

C-6*His

P51608-1 (M1-S486)

4204  [NCBI]

Full Length of Isoform-1

MVAGMLGLREEKSEDQDLQGLKDKPLKFKKVKKDKKEEKEGKHEPVQPSAHHSAEPAEAGKAETSEGSGSAPAVPEASASPKQRRSIIRDRGPMYDDPTLPEGWTRKLKQRKSGRSAGKYDVYLINPQGKAFRSKVELIAYFEKVGDTSLDPNDFDFTVTGRGSPSRREQKPPKKPKSPKAPGTGRGRGRPKGSGTTRPKAATSEGVQVKRVLEKSPGKLLVKMPFQTSPGGKAEGGGATTSTQVMVIKRPGRKRKAEADPQAIPKKRGRKPGSVVAAAAAEAKKKAVKESSIRSVQETVLPIKKRKTRETVSIEVKEVVKPLLVSTLGEKSGKGLKTCKSPGRKSKESSPKGRSSSASSPPKKEHHHHHHHSESPKAPVPLLPPLPPPPPEPESSEDPTSPPEPQDLSSSVCKEEKMPRGGSLESDGCPKEPAKTQPAVATAATAAEKYKHRGEGERKDIVSSSMPRPNREEPVDSRTPVTERVS

Approximately 83.0-90.0 kDa

≥ 90%, as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris, 300 mM NaCl, 5% Trehalose, 2 mM DTT, pH 8.0 or 20 mM Histidine-HCl, 8% Sucrose, 50mM NaCl, 0.02% Tween 80, pH 6.0
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.