MeCP2 Protein, Human (HEK293, His)
Based on 1 publication(s) in Google Scholar
The MeCP2 protein is a chromosome binder that specifically binds to methylated DNA independent of flanking sequences. MeCP2 Protein, Human (HEK293, His) is the recombinant human-derived MeCP2 protein, expressed by HEK293 , with C-6*His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The MeCP2 protein is a chromosome binder that specifically binds to methylated DNA independent of flanking sequences. MeCP2 Protein, Human (HEK293, His) is the recombinant human-derived MeCP2 protein, expressed by HEK293 , with C-6*His labeled tag.
Background
The MeCP2 protein functions as a chromosomal entity with a distinctive ability to bind specifically to methylated DNA, targeting individual methyl-CpG pairs independently of flanking DNA sequences. Operating as a mediator of transcriptional repression, MeCP2 engages in interactions with histone deacetylase and the corepressor SIN3A. Moreover, it demonstrates dual binding capabilities, associating with both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, exhibiting a notable preference for 5-methylcytosine (5mC). The versatility of MeCP2 is further emphasized by its interactions with FNBP3, CDKL5, ATRX, NCOR2, TBL1XR1, and TBL1X, each interaction contributing to various cellular processes, including the recruitment of co-repressor complexes and the orchestration of heterochromatin organization. This multifaceted functionality underscores the pivotal role of MeCP2 in epigenetic regulation and the modulation of gene expression.
Publications (1)
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Journal Impact Factor
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Most Recent
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J Cell Physiol
Critical role of apoptosis in MeCP2-mediated epithelial-mesenchymal transition of ARPE-19 cells. [Abstract]2024 Dec;239(12):e31429. PMID: 39238182
Technical Parameters
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Species Human
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Source HEK293
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Tag C-6*His
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Accession
P51608-1 (M1-S486)
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Molecular Construction
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N-term
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MeCP2 (M1-S486)
Accession # P51608-1 -
6*His
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C-term
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Protein Length
Full Length of Isoform-1
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Synonyms
MECP2; Methyl CpG Binding Protein 2 Isoform 8; Prev. MRX16; Mental Retardation, X-Linked 16; Prev. MRX79; Mental Retardation, X-Linked 79; Prev. RTT; Rett Syndrome; Methyl-CpG-Binding Protein 2; AUTSX3; MeCp-2 Protein; MRXS13; Methyl CpG Binding Protein 2
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AA Sequence
MVAGMLGLREEKSEDQDLQGLKDKPLKFKKVKKDKKEEKEGKHEPVQPSAHHSAEPAEAGKAETSEGSGSAPAVPEASASPKQRRSIIRDRGPMYDDPTLPEGWTRKLKQRKSGRSAGKYDVYLINPQGKAFRSKVELIAYFEKVGDTSLDPNDFDFTVTGRGSPSRREQKPPKKPKSPKAPGTGRGRGRPKGSGTTRPKAATSEGVQVKRVLEKSPGKLLVKMPFQTSPGGKAEGGGATTSTQVMVIKRPGRKRKAEADPQAIPKKRGRKPGSVVAAAAAEAKKKAVKESSIRSVQETVLPIKKRKTRETVSIEVKEVVKPLLVSTLGEKSGKGLKTCKSPGRKSKESSPKGRSSSASSPPKKEHHHHHHHSESPKAPVPLLPPLPPPPPEPESSEDPTSPPEPQDLSSSVCKEEKMPRGGSLESDGCPKEPAKTQPAVATAATAAEKYKHRGEGERKDIVSSSMPRPNREEPVDSRTPVTERVS
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Predicted Molecular Mass
53.5 kDa
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Molecular Weight
Approximately 82-90 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
1.Lyophilized from a 0.22 μm filtered solution of 20 mM Tris, 300 mM NaCl, 5% trehalose, 2 mM DTT, pH 8.0.
2.Lyophilized from a 0.22 μm filtered solution of 20 mM Histidine-HCl, 8% sucrose, 50 mM NaCl, 0.02% Tween 80, pH 6.0.
Please refer to the lot-specific COA for specific buffer information.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (238 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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