1. Recombinant Proteins
  2. Viral Proteins
  3. MERS-CoV Spike/S1 Protein (HEK293, His)

MERS-CoV Spike/S1 Protein (HEK293, His)

Cat. No.: HY-P74751
COA Handling Instructions

The SARS-CoV Spike glycoprotein (S) has three subunits S1, S2' and S2 through alternative splicing. The engagement of the MERS-CoV spike protein S1 with CD26 (also known as dipeptidyl peptidase 4, DPP4) mediates viral attachment to host cells and virus鈥揷ell fusion, thereby initiating infection. S2 mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein and S2' is unmasked following S2 cleavage occurring upon virus endocytosis. MERS-CoV Spike/S1 Protein (HEK293, His) is the recombinant Virus-derived MERS-CoV Spike/S1 protein, expressed by HEK293 , with C-His labeled tag. The total length of MERS-CoV Spike/S1 Protein (HEK293, His) is 725 a.a., with molecular weight of ~94 kDa.

For research use only. We do not sell to patients.

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  • Biological Activity

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Description

The SARS-CoV Spike glycoprotein (S) has three subunits S1, S2' and S2 through alternative splicing. The engagement of the MERS-CoV spike protein S1 with CD26 (also known as dipeptidyl peptidase 4, DPP4) mediates viral attachment to host cells and virus鈥揷ell fusion, thereby initiating infection. S2 mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein and S2' is unmasked following S2 cleavage occurring upon virus endocytosis. MERS-CoV Spike/S1 Protein (HEK293, His) is the recombinant Virus-derived MERS-CoV Spike/S1 protein, expressed by HEK293 , with C-His labeled tag. The total length of MERS-CoV Spike/S1 Protein (HEK293, His) is 725 a.a., with molecular weight of ~94 kDa.

Background

The MERS-CoV Spike glycoprotein (S) has three subunits S1, S2' and S2 through alternative splicing. S1 can attaches the virion to the cell membrane by interacting with host receptor, initiating the infection. S2' acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis. S2 mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein.
Under the current model, S protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.
The engagement of the MERS-CoV spike protein S1 with CD26 (also known as dipeptidyl peptidase 4, DPP4) mediates viral attachment to host cells and virus–cell fusion, thereby initiating infection[1].

Biological Activity

Measured by its binding ability in a functional ELISA. Immobilized Spike Protein S1 at 2 μg/mL (100 μL/well) can bind biotinylated DPP4-Fc and the EC50 is 15-60 ng/mL.

Species

Virus

Source

HEK293

Tag

C-His

Accession

AFS88936 (M1-E725)

Gene ID

14254594  [NCBI]

Molecular Construction
N-term
MERS-CoV S1 (M1-E725)
Accession # AFS88936
His
C-term
Synonyms
Spike glycoprotein; S glycoprotein; E2; Peplomer protein; S
Molecular Weight

Approximately 94 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE

Appearance

Solution.

Formulation

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

N/A.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

MERS-CoV Spike/S1 Protein (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
MERS-CoV Spike/S1 Protein (HEK293, His)
Cat. No.:
HY-P74751
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