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Mesothelin Protein, Human (303a.a, HEK293, His)

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Based on 1 Customer Validation

Mesothelin is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein, as cells overexpressing mesothelin have altered adhesive properties. Mesothelin is also a Megakaryocyte-potentiating factor (MPF) which potentiates megakaryocyte colony formation in vitro. Mesothelin may be related to cancer metastasis. Mesothelin Protein, Human (303a.a, HEK293, His) is the recombinant human-derived Mesothelin protein, expressed by HEK293 , with C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Mesothelin Protein, Human (303a.a, HEK293, His)
  • Experimental Validation Results for Mesothelin Protein, Human (303a.a, HEK293, His)
  • Mesothelin Protein, Human (303a.a, HEK293, His) Product Image
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Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

Mesothelin is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein, as cells overexpressing mesothelin have altered adhesive properties. Mesothelin is also a Megakaryocyte-potentiating factor (MPF) which potentiates megakaryocyte colony formation in vitro. Mesothelin may be related to cancer metastasis. Mesothelin Protein, Human (303a.a, HEK293, His) is the recombinant human-derived Mesothelin protein, expressed by HEK293 , with C-6*His labeled tag.

Background

Mesothelin is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein, as cells overexpressing mesothelin have altered adhesive properties. Mesothelin is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants of Mesothelin. Mesothelin is also a Megakaryocyte-potentiating factor (MPF) which potentiates megakaryocyte colony formation in vitro[1][2].

Verified Bioactivity

1. Loaded Amatuximab (HY-P99006) on AHC2 biosensor, can bind Mesothelin Protein, Human (303a.a, HEK293, His) with an affinity constant of 6.469E-10 M as determined in BLI assay.
2. Loaded HPN536 (HY-P991088) on SA biosensor, can bind Mesothelin Protein, Human (303a.a, HEK293, His) with an affinity constant of 4.442E-10 M as determined in BLI assay.
3.Immobilized Recombinant Human Mesothelin (C-6His) at 2 μg/ml (100 μl/well) can bind Anti-Human Mesothelin mAb. The ED50 of Anti-Human Mesothelin mAb is 1.0-10.0 ng/mL.

MCE Validation Data

  • Bioactivity - BLI

    Bioactivity - BLI

    Loaded Amatuximab (HY-P99006) on AHC2 biosensor, can bind Mesothelin Protein, Human (303a.a, HEK293, His) with an affinity constant of 6.469E-10 M as determined in BLI assay.

  • Bioactivity - BLI

    Bioactivity - BLI

    Loaded HPN536 (HY-P991088) on SA biosensor, can bind Mesothelin Protein, Human (303a.a, HEK293, His) with an affinity constant of 4.442E-10 M as determined in BLI assay.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-6*His
  • Accession

    AAH09272.1 (E296-S598)

  • Gene ID
    10232  [NCBI]
  • Molecular Construction
    • N-term
    • Mesothelin (E296-S598)
      Accession # AAH09272.1
    • 6*His
    • C-term
  • Protein Length

    Full Length of Mesothelin, cleaved form Chain

  • Synonyms

    MSLN; CAK1; Mesothelin; Soluble MPF Mesothelin Related Protein; MPF; Megakaryocyte Potentiating Factor; Pre-Pro-Megakaryocyte-Potentiating Factor; SMRP; CAK1 Antigen

  • AA Sequence

    EVEKTACPSGKKAPEIDESLIFYKKWELEACVDAALLATQMDRVNAIPFTYEQLDVLKHKLDELYPQGYPESVIQHLGYLFLKMSPEDIRKWNVTSLETLKALLEVNKGHEMSPQVATLIDRFVKGRGQLDKDTLDTLTAFYPGYLCSLSPEELSSVPPSSIWAVRPQDLDTCDPRQLDVLYPKARLAFQNMNGSEYFVKIQSFLGGAPTEDLKALSQQNVSMDLATFMKLRTDAVLPLTVAEVQKLLGPHVEGLKAEERHRPVRDWILRQRQDDLDTLGLGLQGGIPNGYLVLDLSMQEALS

  • Predicted Molecular Mass

    34.9 kDa

  • Molecular Weight

    Approximately 35-50 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
2.Lyophilized from a 0.22 μm filtered solution of 20 mM Hepes-NaOH, 50 mM NaCl, 8% trehalose, 0.05% Tween 80, pH 8.0.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Loaded Amatuximab (HY-P99006) on AHC2 biosensor, can bind Mesothelin Protein, Human (303a.a, HEK293, His) with an affinity constant of 6.469E-10 M as determined in BLI assay.

  • 1/16

    MedChemExpress Validation
     Loaded HPN536 (HY-P991088) on SA biosensor, can bind Mesothelin Protein, Human (303a.a, HEK293, His) with an affinity constant of 4.442E-10 M as determined in BLI assay.

  • Mesothelin Protein, Human (303a.a, HEK293, His) Product Image
    1/16
  • Experimental Validation Results for Mesothelin Protein, Human (303a.a, HEK293, His)
  • Experimental Validation Results for Mesothelin Protein, Human (303a.a, HEK293, His)
  • Mesothelin Protein, Human (303a.a, HEK293, His) Product Image