2. Recombinant Proteins
  3. Enzymes & Regulators Kinases
  4. Transferases (EC 2) Serine/Threonine Kinase Proteins Pseudokinases TKL
  5. MLK
  6. MLK1 Protein, Human (Active, Biotinylated, sf9, His, Avi)

MLK1 Protein, Human (Active, Biotinylated, sf9, His, Avi)

Cat. No.: HY-P705605
Handling Instructions Technical Support

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

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Top Publications Citing Use of Products

  • Technical Parameters

  • Properties

  • Documentation

Species

Human
Source

Sf9 insect cells
Tag

C-Avi;N-6*His
Accession

P80192 (Y110-L422)
Gene ID

4293
Molecular Construction
N-term
6*His
MAP3K9 (Y110-L422)
Accession # P80192
Avi
C-term
Protein Length

Partial
Conjugation

Biotin
Conjugate Method

The single lysine residue in Avitag is biotinylated through an enzymatic reaction.
Synonyms
Mitogen-activated protein kinase kinase kinase 9, MAP3K9, PRKE1
AA Sequence

YVTPRSAFSSRCQPGGEDPSCYPPIQLLEIDFAELTLEEIIGIGGFGKVYRAFWIGDEVAVKAARHDPDEDISQTIENVRQEAKLFAMLKHPNIIALRGVCLKEPNLCLVMEFARGGPLNRVLSGKRIPPDILVNWAVQIARGMNYLHDEAIVPIIHRDLKSSNILILQKVENGDLSNKILKITDFGLAREWHRTTKMSAAGTYAWMAPEVIRASMFSKGSDVWSYGVLLWELLTGEVPFRGIDGLAVAYGVAMNKLALPIPSTCPEPFAKLMEDCWNPDPHSRPSFTNILDQLTTIEESGFFEMPKDSFHCL

Predicted Molecular Mass
38.0 kDa
Purity

≥ 95%, as determined by reducing SDS-PAGE.
Documentation
Handling Instructions (2659 KB)

MLK1 Protein, Human (Active, Biotinylated, sf9, His, Avi) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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