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MRE11A Protein, Human (His, GST)

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The MRE11A protein is an important component of the DNA double-strand break repair mechanism and participates in the homologous recombination (HR) pathway. It interacts with RAD50 and NBS1 to form the MRN complex, which is essential for sensing and repairing DNA damage. MRE11A Protein, Human (His, GST) is the recombinant human-derived MRE11A protein, expressed by E. coli , with N-6*His, N-GST labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

The MRE11A protein is an important component of the DNA double-strand break repair mechanism and participates in the homologous recombination (HR) pathway. It interacts with RAD50 and NBS1 to form the MRN complex, which is essential for sensing and repairing DNA damage. MRE11A Protein, Human (His, GST) is the recombinant human-derived MRE11A protein, expressed by E. coli , with N-6*His, N-GST labeled tag.

Background

CXADR, a key component of the epithelial apical junction complex, functions as a homophilic cell adhesion molecule crucial for maintaining tight junction integrity. Additionally, CXADR plays a pivotal role in mediating the transepithelial migration of leukocytes through adhesive interactions with JAML, a transmembrane protein on the plasma membrane of leukocytes. This interaction not only facilitates leukocyte migration but also activates gamma-delta T-cells, a specialized T-cell subpopulation residing in epithelial tissues involved in tissue homeostasis and repair. Upon binding to epithelial CXADR, JAML initiates downstream signaling in gamma-delta T-cells through PI3-kinase and MAP kinases, leading to T-cell proliferation and the production of cytokines and growth factors. These factors, in turn, stimulate the repair processes in epithelial tissues. Notably, CXADR also acts as a receptor for adenovirus type C, highlighting its role in microbial infection.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag N-6*His;N-GST
  • Accession

    P49959 (M1-E411)

  • Gene ID
    4361
  • Molecular Construction
    • N-term
    • 6*His-GST
    • MRE11A (M1-E411)
      Accession # P49959
    • C-term
  • Protein Length

    Partial

  • Synonyms

    MRE11; MRE11 Meiotic Recombination 11 Homolog A (S. Cerevisiae); Prev. MRE11A; MRE11 Meiotic Recombination 11-Like Protein A; MRE11 Homolog, Double Strand Break Repair Nuclease; MRE11 Double Strand Break Repair Nuclease A; ATLD; Double-Strand Break Repair

  • AA Sequence

    MSTADALDDENTFKILVATDIHLGFMEKDAVRGNDTFVTLDEILRLAQENEVDFILLGGDLFHENKPSRKTLHTCLELLRKYCMGDRPVQFEILSDQSVNFGFSKFPWVNYQDGNLNISIPVFSIHGNHDDPTGADALCALDILSCAGFVNHFGRSMSVEKIDISPVLLQKGSTKIALYGLGSIPDERLYRMFVNKKVTMLRPKEDENSWFNLFVIHQNRSKHGSTNFIPEQFLDDFIDLVIWGHEHECKIAPTKNEQQLFYISQPGSSVVTSLSPGEAVKKHVGLLRIKGRKMNMHKIPLHTVRQFFMEDIVLANHPDIFNPDNPKVTQAIQSFCLEKIEEMLENAERERLGNSHQPEKPLVRLRVDYSGGFEPFSVLRFSQKFVDRVANPKDIIHFFRHREQKEKTGEE

  • Predicted Molecular Mass

    74.4 kDa

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution.

Formulation

Supplied as a 0.22 μm filtered solution of 20 mM HEPES, 200 mM NaCl, 2 mM DTT, 0.5 mM MnCl2, 20% glycerol, pH 7.5.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

Please use rapid thawing with running water to thaw the protein.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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