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  2. CAR-T Related Proteins CD Antigens Biotinylated Proteins
  3. MUC-1/CD227 Epithelial cell CD Proteins
  4. Mucin-1/MUC1 Protein, Human (Biotinylated, HEK293, His-Avi)

Mucin-1/MUC1 Protein, Human (Biotinylated, HEK293, His-Avi)

Cat. No.: HY-P78833
COA Handling Instructions

Mucin-1/MUC1 protein and its α subunit have dual functions of adhesion and anti-adhesion proteins, forming a protective layer on epithelial cells. At the same time, the β subunit and its C-terminal domain participate in cell signaling through phosphorylation and protein-protein interactions. Mucin-1/MUC1 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived Mucin-1/MUC1 protein, expressed by HEK293 , with C-Avi, C-His labeled tag. The total length of Mucin-1/MUC1 Protein, Human (Biotinylated, HEK293, His-Avi) is 269 a.a., with molecular weight of 45-70 kDa.

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  • Biological Activity

  • Technical Parameters

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Description

Mucin-1/MUC1 protein and its α subunit have dual functions of adhesion and anti-adhesion proteins, forming a protective layer on epithelial cells. At the same time, the β subunit and its C-terminal domain participate in cell signaling through phosphorylation and protein-protein interactions. Mucin-1/MUC1 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived Mucin-1/MUC1 protein, expressed by HEK293 , with C-Avi, C-His labeled tag. The total length of Mucin-1/MUC1 Protein, Human (Biotinylated, HEK293, His-Avi) is 269 a.a., with molecular weight of 45-70 kDa.

Background

The Mucin-1/MUC1 protein exhibits diverse functional roles: the alpha subunit possesses cell adhesive properties and functions as both an adhesion and an anti-adhesion protein, potentially forming a protective layer on epithelial cells against bacterial and enzyme attacks. Simultaneously, the beta subunit, with its C-terminal domain, engages in cell signaling through phosphorylations and protein-protein interactions. Mucin-1/MUC1 modulates signaling in ERK, SRC, and NF-kappa-B pathways, influencing the Ras/MAPK pathway in activated T-cells. Additionally, it plays a role in promoting tumor progression, regulating TP53-mediated transcription, and determining cell fate in the genotoxic stress response. Notably, in conjunction with KLF4, Mucin-1/MUC1 binds to the PE21 promoter element of TP53, thereby repressing TP53 activity and contributing to the intricate network of cellular functions.

Biological Activity

Measured by its binging ability in a functional ELISA. Immobilized Human MUC1 at 2 μg/mL on streptavidin coated plates can bind Anti-MUC1 antibody. The EC50 is 2.332-4.052 ng/mL.

Species

Human

Source

HEK293

Tag

C-Avi;C-His

Accession

P15941 (S890-G1158)

Gene ID
Synonyms
Mucin 1; MUC1; CD227; EMA; H23AG; KL-6; MAM6; MUC-1; SEC; MUC-1; X; MUC1; ZD; PEM; PEMT; PUM; CA15-3; Episialin
Molecular Weight

45-70 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized a 0.22 μm filtered solution of PBS, 6% Trehalose, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Mucin-1/MUC1 Protein, Human (Biotinylated, HEK293, His-Avi) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Mucin-1/MUC1 Protein, Human (Biotinylated, HEK293, His-Avi)
Cat. No.:
HY-P78833
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