Muellerian-inhibiting factor/AMH Protein, Human (HEK293, His, solution)
Based on 1 Customer Validation
The Müllerian inhibitor/AMH protein plays a crucial role in reproductive processes. It induces degeneration of Müllerian ducts during male fetal sex differentiation and contributes to Leydig cell function. Muellerian-inhibiting factor/AMH Protein, Human (HEK293, His, solution) is the recombinant human-derived Muellerian-inhibiting factor/AMH protein, expressed by HEK293 , with N-His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The Müllerian inhibitor/AMH protein plays a crucial role in reproductive processes. It induces degeneration of Müllerian ducts during male fetal sex differentiation and contributes to Leydig cell function. Muellerian-inhibiting factor/AMH Protein, Human (HEK293, His, solution) is the recombinant human-derived Muellerian-inhibiting factor/AMH protein, expressed by HEK293 , with N-His labeled tag.
Background
The Muellerian-inhibiting factor/AMH protein plays a crucial role in various reproductive functions. Specifically, it induces regression of the Muellerian ducts during male fetal sexual differentiation. It also contributes to Leydig cell differentiation and function in a similar manner. In females, it acts as a negative regulator of the transition from primordial to primary follicles and reduces the sensitivity of growing follicles to follicle-stimulating hormone. AMH exerts its signals by binding to the specific type-II receptor, AMHR2, which forms a heterodimer with type-I receptors (ACVR1 and BMPR1A). This complex recruits SMAD proteins, facilitating their translocation to the nucleus to regulate the expression of target genes. AMH itself forms homodimers through disulfide linkages.
Verified Bioactivity
1.Measured by its binding ability in a functional ELISA. When Recombinant Human Muellerian-inhibiting factor is immobilized at 3 µg/mL(100 µL/well) can bind Recombinant Human AMHRII. The ED50 for this effect is 93.88 ng/mL.
2.Immobilized Recombinant Human AMH / MIF / MIS Protein (His Tag) at 5 μg/mL (100 μL/well) can bind Recombinant Human AMHR2 Protein (Fc Tag), the EC50 is 300-900 ng/mL.
Technical Parameters
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Species Human
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Source HEK293
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Tag N-His
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Accession
NP_000470.2 (L25-R560)
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Gene ID268
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Molecular Construction
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N-term
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His
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AMH (L25-R560)
Accession # P03971 -
C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
AMH; MIS; Anti-Mullerian Hormone; Anti-Muellerian Hormone; Muellerian-Inhibiting Substance; Mullerian Inhibiting Substance; Muellerian-Inhibiting Factor; Mullerian Inhibiting Factor; MIF
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Predicted Molecular Mass
59.1 kDa
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Molecular Weight
Approximately 35 kDa & 40 kDa & 64 kDa, based on SDS-PAGE under reducing conditions.
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Glycosylation
Yes
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.22 μm filtered solution of 20 mM Tris, 150 mM NaCl, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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