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Muellerian-inhibiting factor/AMH Protein, Human (HEK293, His)

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Based on 1 publication(s) in Google Scholar

The Müllerian inhibitor/AMH protein plays a crucial role in reproductive processes. It induces degeneration of Müllerian ducts during male fetal sex differentiation and contributes to Leydig cell function. Muellerian-inhibiting factor/AMH Protein, Human (HEK293, His) is the recombinant human-derived Muellerian-inhibiting factor/AMH protein, expressed by HEK293 , with N-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Muellerian-inhibiting factor/AMH Protein, Human (HEK293, His)
  • Muellerian-inhibiting factor/AMH Protein, Human (HEK293, His) Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

The Müllerian inhibitor/AMH protein plays a crucial role in reproductive processes. It induces degeneration of Müllerian ducts during male fetal sex differentiation and contributes to Leydig cell function. Muellerian-inhibiting factor/AMH Protein, Human (HEK293, His) is the recombinant human-derived Muellerian-inhibiting factor/AMH protein, expressed by HEK293 , with N-His labeled tag.

Background

The Muellerian-inhibiting factor/AMH protein plays a crucial role in various reproductive functions. Specifically, it induces regression of the Muellerian ducts during male fetal sexual differentiation. It also contributes to Leydig cell differentiation and function in a similar manner. In females, it acts as a negative regulator of the transition from primordial to primary follicles and reduces the sensitivity of growing follicles to follicle-stimulating hormone. AMH exerts its signals by binding to the specific type-II receptor, AMHR2, which forms a heterodimer with type-I receptors (ACVR1 and BMPR1A). This complex recruits SMAD proteins, facilitating their translocation to the nucleus to regulate the expression of target genes. AMH itself forms homodimers through disulfide linkages.

Verified Bioactivity

Measured by its binding ability in a functional ELISA. When Recombinant Human Muellerian-inhibiting factor is immobilized at 3 µg/mL(100 µL/well) can bind Recombinant Human AMHRII. The ED50 for this effect is 93.88 ng/mL.

MCE Validation Data

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. When Recombinant Human Muellerian-inhibiting factor is immobilized at 3 µg/mL (100 µL/well) can bind Recombinant Human AMHRII. The ED50 for this effect is 93.88 ng/mL.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag N-His
  • Accession

    NP_000470.2 (L25-R560)

  • Gene ID
    268  [NCBI]
  • Molecular Construction
    • N-term
    • His
    • AMH (L25-R560)
      Accession # P03971
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    AMH; MIS; Anti-Mullerian Hormone; Anti-Muellerian Hormone; Muellerian-Inhibiting Substance; Mullerian Inhibiting Substance; Muellerian-Inhibiting Factor; Mullerian Inhibiting Factor; MIF

  • AA Sequence

    LRAEEPAVGTSGLIFREDLDWPPGSPQEPLCLVALGGDSNGSSSPLRVVGALSAYEQAFLGAVQRARWGPRDLATFGVCNTGDRQAALPSLRRLGAWLRDPGGQRLVVLHLEEVTWEPTPSLRFQEPPPGGAGPPELALLVLYPGPGPEVTVTRAGLPGAQSLCPSRDTRYLVLAVDRPAGAWRGSGLALTLQPRGEDSRLSTARLQALLFGDDHRCFTRMTPALLLLPRSEPAPLPAHGQLDTVPFPPPRPSAELEESPPSADPFLETLTRLVRALRVPPARASAPRLALDPDALAGFPQGLVNLSDPAALERLLDGEEPLLLLLRPTAATTGDPAPLHDPTSAPWATALARRVAAELQAAAAELRSLPGLPPATAPLLARLLALCPGGPGGLGDPLRALLLLKALQGLRVEWRGRDPRGPGRAQRSAGATAADGPCALRELSVDLRAERSVLIPETYQANNCQGVCGWPQSDRNPRYGNHVVLLLKMQARGAALARPPCCVPTAYAGKLLISLSEERISAHHVPNMVATECGCR

  • Predicted Molecular Mass

    59.1 kDa

  • Molecular Weight

    Approximately 64 kDa & 41 kDa & 35 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris, 150 mM NaCl, pH 8.0, 5% trehalose, 5% mannitol and 0.01% Tween 80.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. When Recombinant Human Muellerian-inhibiting factor is immobilized at 3 µg/mL (100 µL/well) can bind Recombinant Human AMHRII. The ED50 for this effect is 93.88 ng/mL.

  • Muellerian-inhibiting factor/AMH Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for Muellerian-inhibiting factor/AMH Protein, Human (HEK293, His)
  • Muellerian-inhibiting factor/AMH Protein, Human (HEK293, His) Product Image