Muellerian-inhibiting factor/AMH Protein, Human (HEK293, His)

The Müllerian inhibitor/AMH protein plays a crucial role in reproductive processes. It induces degeneration of Müllerian ducts during male fetal sex differentiation and contributes to Leydig cell function. Muellerian-inhibiting factor/AMH Protein, Human (HEK293, His) is the recombinant human-derived Muellerian-inhibiting factor/AMH protein, expressed by HEK293 , with N-His labeled tag. The total length of Muellerian-inhibiting factor/AMH Protein, Human (HEK293, His) is 536 a.a., with molecular weight of ~59.1 kDa.

The Muellerian-inhibiting factor/AMH protein plays a crucial role in various reproductive functions. Specifically, it induces regression of the Muellerian ducts during male fetal sexual differentiation. It also contributes to Leydig cell differentiation and function in a similar manner. In females, it acts as a negative regulator of the transition from primordial to primary follicles and reduces the sensitivity of growing follicles to follicle-stimulating hormone. AMH exerts its signals by binding to the specific type-II receptor, AMHR2, which forms a heterodimer with type-I receptors (ACVR1 and BMPR1A). This complex recruits SMAD proteins, facilitating their translocation to the nucleus to regulate the expression of target genes. AMH itself forms homodimers through disulfide linkages.

Human

HEK293

N-His

NP_000470.2 (L25-R560)

268  [NCBI]

Approximately 59.1 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 150 mM NaCl, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.