MVK Protein, Human (sf9, His-GST)

MVK (mevalonate kinase) protein plays a vital role in cellular metabolism by catalyzing the phosphorylation of mevalonate to mevalonate 5-phosphate, which is responsible for isoprenoid and cholesterol biosynthesis. key step. MVK regulates pathways necessary for the synthesis of important cellular components. MVK Protein, Human (sf9, His-GST) is the recombinant human-derived MVK protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.

The MVK protein plays a pivotal role in isoprenoid and cholesterol biosynthesis by catalyzing the phosphorylation of mevalonate to mevalonate 5-phosphate. This enzymatic activity represents a crucial and rate-limiting step in the mevalonate pathway, a metabolic pathway essential for the synthesis of various important molecules, including cholesterol and isoprenoid compounds. The phosphorylation of mevalonate by MVK is a tightly regulated process and a key regulatory point in the overall control of cholesterol and isoprenoid production within cells. Dysregulation of MVK activity has been associated with certain metabolic disorders, emphasizing the significance of this enzyme in maintaining cellular homeostasis and lipid metabolism.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

Sf9 insect cells

N-His;N-GST

Q03426 (M1-L396)

4598  [NCBI]

Full Length

MLSEVLLVSAPGKVILHGEHAVVHGKVALAVSLNLRTFLRLQPHSNGKVDLSLPNIGIKRAWDVARLQSLDTSFLEQGDVTTPTSEQVEKLKEVAGLPDDCAVTERLAVLAFLYLYLSICRKQRALPSLDIVVWSELPPGAGLGSSAAYSVCLAAALLTVCEEIPNPLKDGDCVNRWTKEDLELINKWAFQGERMIHGNPSGVDNAVSTWGGALRYHQGKISSLKRSPALQILLTNTKVPRNTRALVAGVRNRLLKFPEIVAPLLTSIDAISLECERVLGEMGEAPAPEQYLVLEELIDMNQHHLNALGVGHASLDQLCQVTRARGLHSKLTGAGGGGCGITLLKPGLEQPEVEATKQALTSCGFDCLETSIGAPGVSIHSATSLDSRVQQALDGL

Approximately 47 kDa

≥ 85%, as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, 2 mM DTT, pH 7.4, 10% glycerol.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.