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MVK Protein, Human (sf9, His-GST)

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MVK (mevalonate kinase) protein plays a vital role in cellular metabolism by catalyzing the phosphorylation of mevalonate to mevalonate 5-phosphate, which is responsible for isoprenoid and cholesterol biosynthesis. key step. MVK regulates pathways necessary for the synthesis of important cellular components. MVK Protein, Human (sf9, His-GST) is the recombinant human-derived MVK protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: Sf9 insect cells
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
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Biological Activity

Description

MVK (mevalonate kinase) protein plays a vital role in cellular metabolism by catalyzing the phosphorylation of mevalonate to mevalonate 5-phosphate, which is responsible for isoprenoid and cholesterol biosynthesis. key step. MVK regulates pathways necessary for the synthesis of important cellular components. MVK Protein, Human (sf9, His-GST) is the recombinant human-derived MVK protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.

Background

The MVK protein plays a pivotal role in isoprenoid and cholesterol biosynthesis by catalyzing the phosphorylation of mevalonate to mevalonate 5-phosphate. This enzymatic activity represents a crucial and rate-limiting step in the mevalonate pathway, a metabolic pathway essential for the synthesis of various important molecules, including cholesterol and isoprenoid compounds. The phosphorylation of mevalonate by MVK is a tightly regulated process and a key regulatory point in the overall control of cholesterol and isoprenoid production within cells. Dysregulation of MVK activity has been associated with certain metabolic disorders, emphasizing the significance of this enzyme in maintaining cellular homeostasis and lipid metabolism.

Verified Bioactivity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Technical Parameters

  • Species Human
  • Source Sf9 insect cells
  • Tag N-His;N-GST
  • Accession

    Q03426 (M1-L396)

  • Gene ID
    4598  [NCBI]
  • Molecular Construction
    • N-term
    • His-GST
    • MVK (M1-L396)
      Accession # Q03426
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    MVK; Mevalonate Kinase (Mevalonic Aciduria); Mevalonate Kinase; Mevalonate Kinase 1; MK; Mevalonic Aciduria; LRBP; POROK3; LH Receptor MRNA-Binding Protein; MVLK

  • AA Sequence

    MLSEVLLVSAPGKVILHGEHAVVHGKVALAVSLNLRTFLRLQPHSNGKVDLSLPNIGIKRAWDVARLQSLDTSFLEQGDVTTPTSEQVEKLKEVAGLPDDCAVTERLAVLAFLYLYLSICRKQRALPSLDIVVWSELPPGAGLGSSAAYSVCLAAALLTVCEEIPNPLKDGDCVNRWTKEDLELINKWAFQGERMIHGNPSGVDNAVSTWGGALRYHQGKISSLKRSPALQILLTNTKVPRNTRALVAGVRNRLLKFPEIVAPLLTSIDAISLECERVLGEMGEAPAPEQYLVLEELIDMNQHHLNALGVGHASLDQLCQVTRARGLHSKLTGAGGGGCGITLLKPGLEQPEVEATKQALTSCGFDCLETSIGAPGVSIHSATSLDSRVQQALDGL

  • Predicted Molecular Mass

    70.2 kDa

  • Molecular Weight

    Approximately 47 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 85%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, 2 mM DTT, pH 7.4, 10% glycerol.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
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ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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