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NAALADL1 Protein, Mouse (HEK293, His)

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Based on 1 Customer Validation

The NAALADL1 protein is an aminopeptidase that efficiently hydrolyzes a variety of substrates, except those with specific amino acid positions. It has lower activity against substrates containing Asp or Glu at the P2' position or Pro at the P3' position. NAALADL1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived NAALADL1 protein, expressed by HEK293 , with N-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for NAALADL1 Protein, Mouse (HEK293, His)
  • Experimental Validation Results for NAALADL1 Protein, Mouse (HEK293, His)
  • NAALADL1 Protein, Mouse (HEK293, His) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

The NAALADL1 protein is an aminopeptidase that efficiently hydrolyzes a variety of substrates, except those with specific amino acid positions. It has lower activity against substrates containing Asp or Glu at the P2' position or Pro at the P3' position. NAALADL1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived NAALADL1 protein, expressed by HEK293 , with N-His labeled tag.

Background

NAALADL1 protein is an aminopeptidase enzyme that exhibits a broad substrate specificity. It can efficiently hydrolyze a wide range of substrates, except for those that have Asp or Glu in the P2' position, or Pro in the P3' position, where its activity is lower. Additionally, NAALADL1 lacks enzymatic activity when substrates contain both Pro in the P3' position and Asp or Glu in the P2' position. Notably, it does not possess carboxypeptidase activity or dipeptidyl-peptidase IV type activity.

Verified Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized Mouse NAALADL1, at 2μg/mL (100μL/well) can bind Anti-NAALADL1 antibody. The ED50 for this effect is 0.2066 μg/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. Immobilized Mouse NAALADL1, at 2 μg/mL (100 μL/well) can bind Anti-NAALADL1 antibody. The ED50 for this effect is 0.2066 μg/mL.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag N-His
  • Accession

    Q7M758 (P29-L745)

  • Gene ID
    381204  [NCBI]
  • Molecular Construction
    • N-term
    • His
    • NAALADL1 (P29-L745)
      Accession # Q7M758
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    NAALADL1; Ileal Dipeptidylpeptidase; N-Acetylated Alpha-Linked Acidic Dipeptidase Like 1; Ileal Peptidase I100; N-Acetylated-Alpha-Linked Acidic Dipeptidase-Like Protein; NAALADase L; Aminopeptidase NAALADL1; NAALADASEL; HILAP; I100; 100 KDa Ileum Brush B

  • AA Sequence

    PKATSPLTSSTSDSQDLDLAILDSVMGQLDASRIRENLRELSKEPHVATSPRDEALVQLLLGRWKDTATGLDSAKTYEYRVLLSFPNAEQPNSVEVVGPNGTTFHSFQPFEKNLTGEQAGPNVLQPYAAYAPPGTPKGLLVYANQGSEEDFKELETQGINLEGTIALTRYGGVGRGAKAINAAKHGVVGVLVYTDPGDINDGKSLPNETFPNSWRLPPSGVERGSYYEYFGDPLTPYLPAHPSSFRLDPHNTSGFPPIPTQPIGFEDARDLLCNLTGTSAPAFWQGALGCEYKLGPGFEPNGSFPAGSEVKVSVHNRLELRTSSNVLGIIQGAVEPDRYVIYGNHRDSWVHGAVDPSSGTAVLLEISRVLGTLLKKGTWRPRRSIIFASWGAEEFGLIGSTEFTEEFLSKLQERTVAYINVDISVFSNATLRAQGTPPVQSVIFSATKEISAPGSSGLSIYDNWIRYTNRTSPVYGLVPSLGTLGAGSDYAAFVHFLGITSMDLAYTYDRSKTSARIYPTYHTAFDTFDYVEKFLDPGFSSHQAVARTAGSVLLRLSDSLFLPLNVSDYSETLQSFLQAAQEALGTQLEKQNISLGPLVTAVANFKAAAASLGEHILTLQKSSPDPLQVRMVNDQLMLLERAFLNPRAFPEERHYSHVLWAPNTASVDTFPGLANAYAKAQEINSGSEAWAEVQRQLSIVVTALEGAAATLVPVADL

  • Molecular Weight

    Approximately 110 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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=
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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. Immobilized Mouse NAALADL1, at 2 μg/mL (100 μL/well) can bind Anti-NAALADL1 antibody. The ED50 for this effect is 0.2066 μg/mL.

  • NAALADL1 Protein, Mouse (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for NAALADL1 Protein, Mouse (HEK293, His)
  • Experimental Validation Results for NAALADL1 Protein, Mouse (HEK293, His)
  • NAALADL1 Protein, Mouse (HEK293, His) Product Image