NAALADL1 Protein, Mouse (HEK293, His)

The NAALADL1 protein is an aminopeptidase that efficiently hydrolyzes a variety of substrates, except those with specific amino acid positions. It has lower activity against substrates containing Asp or Glu at the P2' position or Pro at the P3' position. NAALADL1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived NAALADL1 protein, expressed by HEK293 , with N-His labeled tag. The total length of NAALADL1 Protein, Mouse (HEK293, His) is 717 a.a., with molecular weight of ~110 kDa.

NAALADL1 protein is an aminopeptidase enzyme that exhibits a broad substrate specificity. It can efficiently hydrolyze a wide range of substrates, except for those that have Asp or Glu in the P2' position, or Pro in the P3' position, where its activity is lower. Additionally, NAALADL1 lacks enzymatic activity when substrates contain both Pro in the P3' position and Asp or Glu in the P2' position. Notably, it does not possess carboxypeptidase activity or dipeptidyl-peptidase IV type activity.

Measured by its binding ability in a functional ELISA. Immobilized Mouse NAALADL1, at 2μg/mL (100μL/well) can bind Anti-NAALADL1 antibody. The ED₅₀ for this effect is 0.2066 μg/mL.

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  Measured by its binding ability in a functional ELISA. Immobilized Mouse NAALADL1, at 2 μg/mL (100 μL/well) can bind Anti-NAALADL1 antibody. The ED₅₀ for this effect is 0.2066 μg/mL.

Mouse

HEK293

N-His

Q7M758 (P29-L745)

381204  [NCBI]

PKATSPLTSSTSDSQDLDLAILDSVMGQLDASRIRENLRELSKEPHVATSPRDEALVQLLLGRWKDTATGLDSAKTYEYRVLLSFPNAEQPNSVEVVGPNGTTFHSFQPFEKNLTGEQAGPNVLQPYAAYAPPGTPKGLLVYANQGSEEDFKELETQGINLEGTIALTRYGGVGRGAKAINAAKHGVVGVLVYTDPGDINDGKSLPNETFPNSWRLPPSGVERGSYYEYFGDPLTPYLPAHPSSFRLDPHNTSGFPPIPTQPIGFEDARDLLCNLTGTSAPAFWQGALGCEYKLGPGFEPNGSFPAGSEVKVSVHNRLELRTSSNVLGIIQGAVEPDRYVIYGNHRDSWVHGAVDPSSGTAVLLEISRVLGTLLKKGTWRPRRSIIFASWGAEEFGLIGSTEFTEEFLSKLQERTVAYINVDISVFSNATLRAQGTPPVQSVIFSATKEISAPGSSGLSIYDNWIRYTNRTSPVYGLVPSLGTLGAGSDYAAFVHFLGITSMDLAYTYDRSKTSARIYPTYHTAFDTFDYVEKFLDPGFSSHQAVARTAGSVLLRLSDSLFLPLNVSDYSETLQSFLQAAQEALGTQLEKQNISLGPLVTAVANFKAAAASLGEHILTLQKSSPDPLQVRMVNDQLMLLERAFLNPRAFPEERHYSHVLWAPNTASVDTFPGLANAYAKAQEINSGSEAWAEVQRQLSIVVTALEGAAATLVPVADL

Approximately 95-125 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.