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NAD kinase/NADK Protein, Human (His)

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NAD kinase (NADK) is located in cytosol, catalyzing the transfer of a phosphate group from ATP to NAD to generate NADP. NADK contributes to cellular redox homeostasis, insulin secretion, cancer regulating, heart disease, anabolic and catabolic processes, neuronal development, and aging. The molecular mechanisms that regulate NADK include protein-protein interaction and post-translational modifications, such as phosphorylation and acetylation including Akt, CaMK and PKC pathways. NAD kinase/NADK Protein, Human (His) is the recombinant human-derived NAD kinase/NADK protein, expressed by E. coli , with N-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
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Biological Activity

Description

NAD kinase (NADK) is located in cytosol, catalyzing the transfer of a phosphate group from ATP to NAD to generate NADP. NADK contributes to cellular redox homeostasis, insulin secretion, cancer regulating, heart disease, anabolic and catabolic processes, neuronal development, and aging. The molecular mechanisms that regulate NADK include protein-protein interaction and post-translational modifications, such as phosphorylation and acetylation including Akt, CaMK and PKC pathways. NAD kinase/NADK Protein, Human (His) is the recombinant human-derived NAD kinase/NADK protein, expressed by E. coli , with N-6*His labeled tag.

Background

NAD kinase (NADK) is located in cytosol, catalyzing the transfer of a phosphate group from ATP to NAD to generate NADP, therefore contributes to cellular redox homeostasis. NADK is also involved in positive regulation of insulin secretion involved in cellular response to glucose stimulus. More over, NADK plays a significant role in regulating cancer, heart disease, anabolic and catabolic processes, neuronal development, and aging.
There are three conserved domains in NADK: the GGDG motif, NE/D short motif and conserved region II. The molecular mechanisms that regulate NADK include protein-protein interaction and post-translational modifications, such as phosphorylation and acetylation including Akt, CaMK and PKC pathways[1][2][3].

Verified Bioactivity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag N-6*His
  • Accession

    O95544-1 (S64-G446)

  • Gene ID
    65220  [NCBI]
  • Molecular Construction
    • N-term
    • 6*His
    • NADK (S64-G446)
      Accession # O95544-1
    • C-term
  • Protein Length

    Partial

  • Synonyms

    NADK; FLJ13052; NAD Kinase; NAD(+) Kinase; Poly(P)/ATP NAD Kinase; DJ283E3.1; NADK1

  • AA Sequence

    SLHGPCPVTTFGPKACVLQNPQTIMHIQDPASQRLTWNKSPKSVLVIKKMRDASLLQPFKELCTHLMEENMIVYVEKKVLEDPAIASDESFGAVKKKFCTFREDYDDISNQIDFIICLGGDGTLLYASSLFQGSVPPVMAFHLGSLGFLTPFSFENFQSQVTQVIEGNAAVVLRSRLKVRVVKELRGKKTAVHNGLGENGSQAAGLDMDVGKQAMQYQVLNEVVIDRGPSSYLSNVDVYLDGHLITTVQGDGVIVSTPTGSTAYAAAAGASMIHPNVPAIMITPICPHSLSFRPIVVPAGVELKIMLSPEARNTAWVSFDGRKRQEIRHGDSISITTSCYPLPSICVRDPVSDWFESLAQCLHWNVRKKQAHFEEEEEEEEEG

  • Predicted Molecular Mass

    44.4 kDa

  • Molecular Weight

    Approximately 49 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 20 mM His-HCl, 5% Sucrose, 10% Glycerol, 150 mM NaCl, 0.02% Tween80, 1 mM DTT, pH 3.0.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
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MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

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