NAD kinase/NADK Protein, Human (His)

NAD kinase (NADK) is located in cytosol, catalyzing the transfer of a phosphate group from ATP to NAD to generate NADP. NADK contributes to cellular redox homeostasis, insulin secretion, cancer regulating, heart disease, anabolic and catabolic processes, neuronal development, and aging. The molecular mechanisms that regulate NADK include protein-protein interaction and post-translational modifications, such as phosphorylation and acetylation including Akt, CaMK and PKC pathways. NAD kinase/NADK Protein, Human (His) is the recombinant human-derived NAD kinase/NADK protein, expressed by E. coli , with N-6*His labeled tag. The total length of NAD kinase/NADK Protein, Human (His) is 383 a.a., with molecular weight of ~49.0 kDa.

NAD kinase (NADK) is located in cytosol, catalyzing the transfer of a phosphate group from ATP to NAD to generate NADP, therefore contributes to cellular redox homeostasis. NADK is also involved in positive regulation of insulin secretion involved in cellular response to glucose stimulus. More over, NADK plays a significant role in regulating cancer, heart disease, anabolic and catabolic processes, neuronal development, and aging.
There are three conserved domains in NADK: the GGDG motif, NE/D short motif and conserved region II. The molecular mechanisms that regulate NADK include protein-protein interaction and post-translational modifications, such as phosphorylation and acetylation including Akt, CaMK and PKC pathways^[1][2][3].

Human

E. coli

N-6*His

O95544 (S64-G446)

65220  [NCBI]

SLHGPCPVTTFGPKACVLQNPQTIMHIQDPASQRLTWNKSPKSVLVIKKMRDASLLQPFKELCTHLMEENMIVYVEKKVLEDPAIASDESFGAVKKKFCTFREDYDDISNQIDFIICLGGDGTLLYASSLFQGSVPPVMAFHLGSLGFLTPFSFENFQSQVTQVIEGNAAVVLRSRLKVRVVKELRGKKTAVHNGLGENGSQAAGLDMDVGKQAMQYQVLNEVVIDRGPSSYLSNVDVYLDGHLITTVQGDGVIVSTPTGSTAYAAAAGASMIHPNVPAIMITPICPHSLSFRPIVVPAGVELKIMLSPEARNTAWVSFDGRKRQEIRHGDSISITTSCYPLPSICVRDPVSDWFESLAQCLHWNVRKKQAHFEEEEEEEEEG

Approximately 49.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.