NADH dehydrogenase Protein, Gluconobacter oxydans

NADH dehydrogenase is a protein complex involved in the electron transport chain of cellular respiration. It catalyzes the transfer of electrons from NADH to coenzyme Q, contributing to the generation of a proton gradient used for ATP synthesis. The protein plays a vital role in energy production within the mitochondria. NADH dehydrogenase Protein, Gluconobacter oxydans is the recombinant NADH dehydrogenase protein, expressed by E. coli , with tag free.

For research use only. We do not sell to patients.
  • Species: Others
  • Source: E. coli
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs

Biological Activity

Description

NADH dehydrogenase is a protein complex involved in the electron transport chain of cellular respiration. It catalyzes the transfer of electrons from NADH to coenzyme Q, contributing to the generation of a proton gradient used for ATP synthesis. The protein plays a vital role in energy production within the mitochondria. NADH dehydrogenase Protein, Gluconobacter oxydans is the recombinant NADH dehydrogenase protein, expressed by E. coli , with tag free.

Background

NADH dehydrogenase is a crucial protein involved in cellular respiration, particularly in the electron transport chain. As the initial enzyme complex of complex I, it catalyzes the transfer of electrons from NADH to ubiquinone, generating a proton gradient across the inner mitochondrial membrane. This gradient is essential for ATP synthesis. NADH dehydrogenase's role highlights its significance in energy production within cells.

Technical Parameters

  • Species Others
  • Source E. coli
  • Tag Tag Free
  • Accession
  • Gene ID
  • Molecular Construction
    • N-term
    • NDH-2 (M1-A409)
      Accession # A0A4R3ZYF0
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    NADH dehydrogenase; EDC20_11241

  • AA Sequence

    MSAGARVLVLGGGVGGLEAAAALGRRQNISLTLVDRSPLHYWKPALHEFAAGTMQHQGNCIPFKDTAVKFGFTFVQGSPVAVNRENKTVSLDTGATLPYDYLVVALGSRANDFGIPGIVDHCMFIDSLNDADALFATFREAVQKARAAGEKLSVGIVGGGATGVQLIAELCKSIDDARGLGPAVRKSLLNAVLIETGPRILPAFPEAVSTAATQQLEKLGISVRTEAMVVGADETGFSLKSGEHIPASLRVWAAGVRASDATNLLEGLERGRSGQLGVTTTLQTTQDPAVFAIGDCARIDAAPIAPTAQAARQQGQYLGRALPEIIAGRTPAPFAYNDRGAVVALGDYNGWGMLDAKRSFGGGLLSGLAARLIHEGLYRQHLAGILGVAPTACSTVREHLSPVKPDLGA

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

Please use rapid thawing with running water to thaw the protein.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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=
Mass (in vial) Mass (in vial)
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Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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