2. Recombinant Proteins
  3. Enzymes & Regulators
  4. Serine/Threonine Kinase Proteins
  5. NIMA Related Kinase 3
  6. NEK3 Protein, Mouse (sf9, His-GST)

NEK3 Protein, Mouse (sf9, His-GST)

Cat. No.: HY-P76509
Handling Instructions

NEK3 Protein influences neuronal morphogenesis and polarity through effects on microtubules. NEK3 Protein regulates microtubule acetylation in neurons. NEK3 Protein contributes to prolactin-mediated phosphorylation of PXN and VAV2. NEK3 Protein, Mouse (sf9, His-GST) is the recombinant mouse-derived NEK3 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of NEK3 Protein, Mouse (sf9, His-GST) is 509 a.a., with molecular weight of ~72 KDa.

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Description

NEK3 Protein influences neuronal morphogenesis and polarity through effects on microtubules. NEK3 Protein regulates microtubule acetylation in neurons. NEK3 Protein contributes to prolactin-mediated phosphorylation of PXN and VAV2. NEK3 Protein, Mouse (sf9, His-GST) is the recombinant mouse-derived NEK3 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of NEK3 Protein, Mouse (sf9, His-GST) is 509 a.a., with molecular weight of ~72 KDa.
Background

NEK3 encodes a member of the NimA family of serine/threonine protein kinases. NEK3 protein differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. Nek3 is activated by prolactin stimulation, leading to phosphorylation of VAV2 guanine nucleotide exchange factor, paxillin, and activation of the RAC1 GTPase[1][2].
Species

Mouse
Source

Sf9 insect cells
Tag

N-His;N-GST
Accession

ABK42288 (M1-A509)
Gene ID

/
Molecular Construction
N-term
His-GST
NEK3 (M1-A509)
Accession # ABK42288
C-term
Synonyms
Serine/threonine-protein kinase Nek3; HSPK 36; NimA-related protein kinase 3
Molecular Weight

Approximately 72 kDa.
Purity

Greater than 90% as determined by reducing SDS-PAGE
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

NEK3 Protein, Mouse (sf9, His-GST) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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