NEK3 Protein, Mouse (sf9, His-GST)
NEK3 Protein influences neuronal morphogenesis and polarity through effects on microtubules. NEK3 Protein regulates microtubule acetylation in neurons. NEK3 Protein contributes to prolactin-mediated phosphorylation of PXN and VAV2. NEK3 Protein, Mouse (sf9, His-GST) is the recombinant mouse-derived NEK3 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.
- Species: Mouse
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
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Biological Activity
Description
NEK3 Protein influences neuronal morphogenesis and polarity through effects on microtubules. NEK3 Protein regulates microtubule acetylation in neurons. NEK3 Protein contributes to prolactin-mediated phosphorylation of PXN and VAV2. NEK3 Protein, Mouse (sf9, His-GST) is the recombinant mouse-derived NEK3 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.
Background
NEK3 encodes a member of the NimA family of serine/threonine protein kinases. NEK3 protein differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. Nek3 is activated by prolactin stimulation, leading to phosphorylation of VAV2 guanine nucleotide exchange factor, paxillin, and activation of the RAC1 GTPase[1][2].
Technical Parameters
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Species Mouse
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Source Sf9 insect cells
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Tag N-His;N-GST
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Accession
ABK42288 (M1-A509)
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Gene ID/
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Molecular Construction
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N-term
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His-GST
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NEK3 (M1-A509)
Accession # ABK42288 -
C-term
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Protein Length
Full Length
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Synonyms
Nek3; Never in mitosis A-related kinase 3 (NimA-related protein kinase 3); HSPK 36; EC:2.7.11.1; Serine/threonine-protein kinase Nek3
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AA Sequence
MDNYTVLRVIGQGSFGRALLVLQESSNQTFAMKEIRLLKSDTQTSRKEAVLLAKMKHPNIVAFKESFEAEGYLYIVMEYCDGGDLMQRIKQQKGNLFPEDTILNWFIQICLGVNHIHKRRVLHRDIKSKNVFLTHNGKVKLGDFGSARLLSSPMAFACTYVGTPYYVPPEIWENLPYNNKSDIWSLGCILYELCALKHPFQANSWKNLILKICQGPIHPLPALYSCKLQGLVKQMLKRNPSHRPSATTLLCRGSLAPLVPKCLPPQIIREYGEQILDEIKISTPKNMKKQDSNRVGRALGEANSAAMQEEERGRKCSHTELESTGTTPAGNALGRAARGNPGNRQEHGSHTSPASPHRPRWERHGPSSNVEALEKASILTSSFTAEDDRGGSVIKYEENARRQWVREPPEALLSMLKDADLSQAFQTYTIYRPGAEGFLKGPLSEDTASDSVDGDLDSVMLDPERFEPRLDEEDTDFEEDNENPDWVSELKKHVGYGDGPGGQLLGERA
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Predicted Molecular Mass
84.8 kDa
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Molecular Weight
Approximately 72 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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