Neogenin Protein, Human (HEK293, hFc)

Neogenin is a multifunctional cell surface receptor that controls multiple developmental processes by regulating cell adhesion. It affects neural tube and mammary gland formation, myogenesis and angiogenesis. Neogenin Protein, Human (HEK293, hFc) is the recombinant human-derived Neogenin protein, expressed by HEK293 , with C-hFc labeled tag.

Neogenin, a versatile cell surface receptor, orchestrates diverse developmental processes by regulating cell adhesion. Its influence spans neural tube and mammary gland formation, myogenesis, and angiogenesis. Functioning as a receptor for members of the BMP, netrin, and repulsive guidance molecule (RGM) families, Neogenin engages in complex interactions pivotal to cellular responses. Notably, interactions with netrin lead to chemoattractive axon guidance responses and cell-cell adhesion, while engagements with RGMa and RGMb induce chemorepulsive responses. The receptor also interacts with MYO10 and forms associations with specific BMPs, including BMP2, BMP4, BMP6, and BMP7. This comprehensive network of interactions underscores Neogenin's central role in regulating cell adhesion across a spectrum of crucial developmental contexts.

Immobilized Human RGMa, His Tag at 5 μg/mL (100 μl/Well) on the plate. Dose response curve for Human Neogenin, hFc Tag with the EC₅₀ of ≤0.5 μg/mL determined by ELISA.

Human

HEK293

C-hFc

Q92859-1 (A34-L1105)

/

Extracellular Domain

ARSGSAPQSPGASIRTFTPFYFLVEPVDTLSVRGSSVILNCSAYSEPSPKIEWKKDGTFLNLVSDDRRQLLPDGSLFISNVVHSKHNKPDEGYYQCVATVESLGTIISRTAKLIVAGLPRFTSQPEPSSVYAGNNAILNCEVNADLVPFVRWEQNRQPLLLDDRVIKLPSGMLVISNATEGDGGLYRCVVESGGPPKYSDEVELKVLPDPEVISDLVFLKQPSPLVRVIGQDVVLPCVASGLPTPTIKWMKNEEALDTESSERLVLLAGGSLEISDVTEDDAGTYFCIADNGNETIEAQAELTVQAQPEFLKQPTNIYAHESMDIVFECEVTGKPTPTVKWVKNGDMVIPSDYFKIVKEHNLQVLGLVKSDEGFYQCIAENDVGNAQAGAQLIILEHAPATTGPLPSAPRDVVASLVSTRFIKLTWRTPASDPHGDNLTYSVFYTKEGIARERVENTSHPGEMQVTIQNLMPATVYIFRVMAQNKHGSGESSAPLRVETQPEVQLPGPAPNLRAYAASPTSITVTWETPVSGNGEIQNYKLYYMEKGTDKEQDVDVSSHSYTINGLKKYTEYSFRVVAYNKHGPGVSTPDVAVRTLSDVPSAAPQNLSLEVRNSKSIMIHWQPPAPATQNGQITGYKIRYRKASRKSDVTETLVSGTQLSQLIEGLDRGTEYNFRVAALTINGTGPATDWLSAETFESDLDETRVPEVPSSLHVRPLVTSIVVSWTPPENQNIVVRGYAIGYGIGSPHAQTIKVDYKQRYYTIENLDPSSHYVITLKAFNNVGEGIPLYESAVTRPHTDTSEVDLFVINAPYTPVPDPTPMMPPVGVQASILSHDTIRITWADNSLPKHQKITDSRYYTVRWKTNIPANTKYKNANATTLSYLVTGLKPNTLYEFSVMVTKGRRSSTWSMTAHGTTFELVPTSPPKDVTVVSKEGKPKTIIVNWQPPSEANGKITGYIIYYSTDVNAEIHDWVIEPVVGNRLTHQIQELTLDTPYYFKIQARNSKGMGPMSEAVQFRTPKADSSDKMPNDQASGSGGKGSRLPDLGSDYKPPMSGSNSPHGSPTSPLDSNML

Approximately 150-180 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.