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Neogenin Protein, Human (HEK293, hFc)

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Neogenin is a multifunctional cell surface receptor that controls multiple developmental processes by regulating cell adhesion. It affects neural tube and mammary gland formation, myogenesis and angiogenesis. Neogenin Protein, Human (HEK293, hFc) is the recombinant human-derived Neogenin protein, expressed by HEK293 , with C-hFc labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Neogenin Protein, Human (HEK293, hFc) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

Neogenin is a multifunctional cell surface receptor that controls multiple developmental processes by regulating cell adhesion. It affects neural tube and mammary gland formation, myogenesis and angiogenesis. Neogenin Protein, Human (HEK293, hFc) is the recombinant human-derived Neogenin protein, expressed by HEK293 , with C-hFc labeled tag.

Background

Neogenin, a versatile cell surface receptor, orchestrates diverse developmental processes by regulating cell adhesion. Its influence spans neural tube and mammary gland formation, myogenesis, and angiogenesis. Functioning as a receptor for members of the BMP, netrin, and repulsive guidance molecule (RGM) families, Neogenin engages in complex interactions pivotal to cellular responses. Notably, interactions with netrin lead to chemoattractive axon guidance responses and cell-cell adhesion, while engagements with RGMa and RGMb induce chemorepulsive responses. The receptor also interacts with MYO10 and forms associations with specific BMPs, including BMP2, BMP4, BMP6, and BMP7. This comprehensive network of interactions underscores Neogenin's central role in regulating cell adhesion across a spectrum of crucial developmental contexts.

Verified Bioactivity

Immobilized Human RGMa, His Tag at 5 μg/mL (100 μl/Well) on the plate. Dose response curve for Human Neogenin, hFc Tag with the EC50 of ≤0.5 μg/mL determined by ELISA.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-hFc
  • Accession

    Q92859-1 (A34-L1105)

  • Gene ID
    /
  • Molecular Construction
    • N-term
    • Neogenin (A34-L1105)
      Accession # Q92859-1
    • hFc
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    NEO1; Immunoglobulin Superfamily DCC Subclass Member 2; Neogenin 1; HsT17534; IGDCC2; Immunoglobulin Superfamily, DCC Subclass, Member 2; NGN; Neogenin (Chicken) Homolog 1; Neogenin; Neogenin Homolog 1; NTN1R2

  • AA Sequence

    ARSGSAPQSPGASIRTFTPFYFLVEPVDTLSVRGSSVILNCSAYSEPSPKIEWKKDGTFLNLVSDDRRQLLPDGSLFISNVVHSKHNKPDEGYYQCVATVESLGTIISRTAKLIVAGLPRFTSQPEPSSVYAGNNAILNCEVNADLVPFVRWEQNRQPLLLDDRVIKLPSGMLVISNATEGDGGLYRCVVESGGPPKYSDEVELKVLPDPEVISDLVFLKQPSPLVRVIGQDVVLPCVASGLPTPTIKWMKNEEALDTESSERLVLLAGGSLEISDVTEDDAGTYFCIADNGNETIEAQAELTVQAQPEFLKQPTNIYAHESMDIVFECEVTGKPTPTVKWVKNGDMVIPSDYFKIVKEHNLQVLGLVKSDEGFYQCIAENDVGNAQAGAQLIILEHAPATTGPLPSAPRDVVASLVSTRFIKLTWRTPASDPHGDNLTYSVFYTKEGIARERVENTSHPGEMQVTIQNLMPATVYIFRVMAQNKHGSGESSAPLRVETQPEVQLPGPAPNLRAYAASPTSITVTWETPVSGNGEIQNYKLYYMEKGTDKEQDVDVSSHSYTINGLKKYTEYSFRVVAYNKHGPGVSTPDVAVRTLSDVPSAAPQNLSLEVRNSKSIMIHWQPPAPATQNGQITGYKIRYRKASRKSDVTETLVSGTQLSQLIEGLDRGTEYNFRVAALTINGTGPATDWLSAETFESDLDETRVPEVPSSLHVRPLVTSIVVSWTPPENQNIVVRGYAIGYGIGSPHAQTIKVDYKQRYYTIENLDPSSHYVITLKAFNNVGEGIPLYESAVTRPHTDTSEVDLFVINAPYTPVPDPTPMMPPVGVQASILSHDTIRITWADNSLPKHQKITDSRYYTVRWKTNIPANTKYKNANATTLSYLVTGLKPNTLYEFSVMVTKGRRSSTWSMTAHGTTFELVPTSPPKDVTVVSKEGKPKTIIVNWQPPSEANGKITGYIIYYSTDVNAEIHDWVIEPVVGNRLTHQIQELTLDTPYYFKIQARNSKGMGPMSEAVQFRTPKADSSDKMPNDQASGSGGKGSRLPDLGSDYKPPMSGSNSPHGSPTSPLDSNML

  • Predicted Molecular Mass

    144.4 kDa

  • Molecular Weight

    Approximately 150-180 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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