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NETO1 Protein, Human (HEK293, His)

Cat. No.: HY-P75937
SDS COA Handling Instructions

The NETO1 protein complexly maintains neuronal circuits as an auxiliary subunit of NMDARs, which is critical for the abundance of GRIN2A-containing NMDARs in the postsynaptic density. It regulates long-term NMDA receptor-dependent synaptic plasticity and cognition, particularly in spatial learning and memory. NETO1 Protein, Human (HEK293, His) is the recombinant human-derived NETO1 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

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  • Documentation

Description

The NETO1 protein complexly maintains neuronal circuits as an auxiliary subunit of NMDARs, which is critical for the abundance of GRIN2A-containing NMDARs in the postsynaptic density. It regulates long-term NMDA receptor-dependent synaptic plasticity and cognition, particularly in spatial learning and memory. NETO1 Protein, Human (HEK293, His) is the recombinant human-derived NETO1 protein, expressed by HEK293 , with C-His labeled tag.

Background

The NETO1 Protein is intricately involved in the development and maintenance of neuronal circuitry. Serving as an accessory subunit of the neuronal N-methyl-D-aspartate receptor (NMDAR), NETO1 plays a critical role in maintaining the abundance of NMDARs containing GRIN2A in the postsynaptic density. Furthermore, NETO1 functions as a regulator of long-term NMDA receptor-dependent synaptic plasticity and cognition, particularly in the context of spatial learning and memory. The protein's interactions extend to the PLZ domains of DLG2, DLG3, and DLG4 through its C-terminal TRV domain, highlighting its involvement in synaptic signaling complexes. Additionally, NETO1 interacts with GRIN2A and GRIN2B via its CUB domains, indicating its role in modulating the composition and function of NMDARs in neuronal synapses.

Biological Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human NETO1 Protein is immobilized at 4.00 µg/mL (100 µL/well), Recombinant Human Amyloid Precursor binds with an ED50 of 0.6861 μg/mL.

  • Measured by its binding ability in a functional ELISA. When Recombinant Human NETO1 Protein is immobilized at 4.00 µg/mL (100 µL/well), Recombinant Human Amyloid Precursor binds with an ED50 of 0.6861 μg/mL.
Species

Human

Source

HEK293

Tag

C-6*His

Accession

Q8TDF5-3 (T23-T344)

Gene ID
Molecular Construction
N-term
NETO1 (T23-T344)
Accession # Q8TDF5-3
His
C-term
Synonyms
Neuropilin and tolloid-like protein 1; NETO1; BTCL1
AA Sequence

TKKGTEKQTTSETQKSVQCGTWTKHAEGGIFTSPNYPSKYPPDRECIYIIEAAPRQCIELYFDEKYSIEPSWECKFDHIEVRDGPFGFSPIIGRFCGQQNPPVIKSSGRFLWIKFFADGELESMGFSARYNFTPDPDFKDLGALKPLPACEFEMGGSEGIVESIQIMKEGKATASEAVDCKWYIRAPPRSKIYLRFLDYEMQNSNECKRNFVAVYDGSSSVEDLKAKFCSTVANDVMLRTGLGVIRMWADEGSRNSRFQMLFTSFQEPPCEGNTFFCHSNMCINNTLVCNGLQNCVYPWDENHCKEKRKTSLLDQLTNTSGT

Molecular Weight

Approximately 40-50 kDa due to the glycosylation

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

NETO1 Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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NETO1 Protein, Human (HEK293, His)
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HY-P75937
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