Neutrophil cytosol factor 1 Protein, Human (His)

Neutrophil cytoplasmic factor 1 (NCF1) is essential for activation of the NADPH oxidase complex, which is essential for superoxide production. It cooperates with NCF2 to form a core association in the complex, interacting with NCF4, CYBB and CYBA. Neutrophil cytosol factor 1 Protein, Human (His) is the recombinant human-derived Neutrophil cytosol factor 1 protein, expressed by E. coli , with C-6*His labeled tag.

Neutrophil cytosol factor 1 (NCF1) is an essential component, along with NCF2 and a membrane-bound cytochrome b558, for the activation of the latent NADPH oxidase, a process crucial for superoxide production. It forms an integral part of the NADPH oxidase complex, which includes the membrane proteins CYBA and CYBB, along with the cytosolic subunits NCF1, NCF2, and NCF4. NCF1 interacts with NCF2 through its C-terminus, forming a key association within the complex. Additionally, it engages with NCF4, CYBB, and CYBA, demonstrating its central role in coordinating the assembly of this complex. Interactions with NOXA1, ADAM15, TRAF4, and FASLG further emphasize the regulatory network of NCF1. Notably, its interaction with PARK7, enhanced by LPS, modulates NCF1 phosphorylation and membrane translocation, adding a layer of complexity to its regulatory functions.

Human

E. coli

C-6*His

P14598 (M1-V390)

653361  [NCBI]

Full Length

MGDTFIRHIALLGFEKRFVPSQHYVYMFLVKWQDLSEKVVYRRFTEIYEFHKTLKEMFPIEAGAINPENRIIPHLPAPKWFDGQRAAENRQGTLTEYCSTLMSLPTKISRCPHLLDFFKVRPDDLKLPTDNQTKKPETYLMPKDGKSTATDITGPIILQTYRAIANYEKTSGSEMALSTGDVVEVVEKSESGWWFCQMKAKRGWIPASFLEPLDSPDETEDPEPNYAGEPYVAIKAYTAVEGDEVSLLEGEAVEVIHKLLDGWWVIRKDDVTGYFPSMYLQKSGQDVSQAQRQIKRGAPPRRSSIRNAHSIHQRSRKRLSQDAYRRNSVRFLQQRRRQARPGPQSPGSPLEEERQTQRSKPQPAVPPRPSADLILNRCSESTKRKLASAV

Approximately 45-50 kDa,based on SDS-PAGE under reducing conditions.

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.