NFKBIE/IKB epsilon Protein, Human (His)
Based on 1 Customer Validation
NFKBIE/IKB epsilon Protein crucially inhibits NF-kappa-B by forming a cytoplasmic complex, preventing its DNA-binding activity. It targets specific complexes involving p50-p65, p50-c-Rel, and interacts with subunits RELA, REL, NFKB1 (p50), and NFKB2 (p52). NFKBIE/IKB epsilon plays a pivotal role in regulating NF-kappa-B signaling, controlling nuclear translocation and transcriptional activities. NFKBIE/IKB epsilon Protein, Human (His) is the recombinant human-derived NFKBIE/IKB epsilon protein, expressed by E. coli , with N-10*His labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
NFKBIE/IKB epsilon Protein crucially inhibits NF-kappa-B by forming a cytoplasmic complex, preventing its DNA-binding activity. It targets specific complexes involving p50-p65, p50-c-Rel, and interacts with subunits RELA, REL, NFKB1 (p50), and NFKB2 (p52). NFKBIE/IKB epsilon plays a pivotal role in regulating NF-kappa-B signaling, controlling nuclear translocation and transcriptional activities. NFKBIE/IKB epsilon Protein, Human (His) is the recombinant human-derived NFKBIE/IKB epsilon protein, expressed by E. coli , with N-10*His labeled tag.
Background
NFKBIE/IKB epsilon protein plays a crucial role in inhibiting NF-kappa-B by forming a complex with it, thereby sequestering it in the cytoplasm. It exerts its inhibitory effects by preventing the DNA-binding activity of NF-kappa-B, specifically targeting complexes involving p50-p65, p50-c-Rel, and interacting with individual subunits such as RELA, REL, NFKB1 (p50), and NFKB2 (p52). Through these interactions, NFKBIE/IKB epsilon contributes to the regulation of NF-kappa-B signaling, playing a pivotal role in controlling its nuclear translocation and subsequent transcriptional activities.
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
Technical Parameters
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Species Human
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Source E. coli
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Tag N-10*His
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Accession
O00221 (M1-D500)
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Gene ID4794
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Molecular Construction
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N-term
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10*His
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NFKBIE (M1-D500)
Accession # O00221 -
C-term
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Protein Length
Full Length
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Synonyms
NFKBIE; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells Inhibitor, Epsilon; NFKB Inhibitor Epsilon; IkappaBepsilon; NF-Kappa-B Inhibitor Epsilon; NF-Kappa-BIE; IKBE; IkB-Epsilon; I-Kappa-B-Epsilon; IkB-E
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AA Sequence
MNQRRSESRPGNHRLQAYAEPGKGDSGGAGPLSGSARRGRGGGGAIRVRRPCWSGGAGRGGGPAWAVRLPTVTAGWTWPALRTLSSLRAGPSEPHSPGRRPPRAGRPLCQADPQPGKAARRSLEPDPAQTGPRPARAAGMSEARKGPDEAEESQYDSGIESLRSLRSLPESTSAPASGPSDGSPQPCTHPPGPVKEPQEKEDADGERADSTYGSSSLTYTLSLLGGPEAEDPAPRLPLPHVGALSPQQLEALTYISEDGDTLVHLAVIHEAPAVLLCCLALLPQEVLDIQNNLYQTALHLAVHLDQPGAVRALVLKGASRALQDRHGDTALHVACQRQHLACARCLLEGRPEPGRGTSHSLDLQLQNWQGLACLHIATLQKNQPLMELLLRNGADIDVQEGTSGKTALHLAVETQERGLVQFLLQAGAQVDARMLNGCTPLHLAAGRGLMGISSTLCKAGADSLLRNVEDETPQDLTEESLVLLPFDDLKISGKLLLCTD
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Predicted Molecular Mass
52.8 kDa
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Molecular Weight
Approximately 57 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of 50 mM Tris-HCl, 200 mM NaCl, 200 mM arginine, pH 8.0, 10% glycerol.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (238 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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