nitA Protein, Rhodococcus rhodochrous
nitA protein enzymatically acts on aliphatic nitriles, specifically acrylonitrile, crotononitrile, and glutaronitrile substrates. nitA Protein, Rhodococcus rhodochrous is the recombinant nitA protein, expressed by E. coli , with tag free.
- Species: Others
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
nitA protein enzymatically acts on aliphatic nitriles, specifically acrylonitrile, crotononitrile, and glutaronitrile substrates. nitA Protein, Rhodococcus rhodochrous is the recombinant nitA protein, expressed by E. coli , with tag free.
Background
nitA protein demonstrates its enzymatic activity on aliphatic nitriles, specifically targeting substrates like acrylonitrile, crotononitrile, and glutaronitrile.
Technical Parameters
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Species Others
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Source E. coli
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Tag Tag Free
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Accession
ABD98457.1 (M1-K369)
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Gene ID/
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Molecular Construction
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N-term
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nitA (M1-K369)
Accession # ABD98457.1 -
C-term
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Protein Length
Partial
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Synonyms
Nitrilase; EC:3.5.5.1; nitA; Aliphatic nitrilase
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AA Sequence
MVSYNSKFLAATVQAEPVWLDADATIDKSIGIIEEAAQKGASLIAFPEVFIPGYPYWAWLGDVKYSLSFTSRYHENSLELGDDRMRRLQLAARRNKIALVMGYSEREAGSRYLSQVFIDERGEIVANRRKLKPTHVERTIYGEGNGTDFLTHDFAFGRVGGLNCWEHFQPLSKFMMYSLGEQVHVASWPAMSPLQPDVFQLSIEANATVTRSYAIEGQTFVLCSTQVIGPSAIETFCLNDEQRALLPQGCGWARIYGPDGSELAKPLAEDAEGILYAEIDLEQILLAKAGADPVGHYSRPDVLSVQFDPRNHTPVHRIGIDGRLDVNTRSRVENFRLRQAAEQERQASKRLGTKLFEQSLLAEEPVPAK
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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