NOV/CCN3 Protein, Canine (HEK293, His)

NOV/CCN3 Protein belongs to an emerging family of growth regulators referred under the CCN acronym. NOV/CCN3 Protein enables heparin and binding. NOV/CCN3 Protein is involved in cell adhesion and signal transduction. NOV/CCN3 Protein, Canine (HEK293, His) is the recombinant canine-derived NOV/CCN3 protein, expressed by HEK293 , with C-His labeled tag. The total length of NOV/CCN3 Protein, Canine (HEK293, His) is 325 a.a., with molecular weight of 42-50 KDa.

NOV/CCN3 Protein belongs to an emerging family of growth regulators referred under the CCN acronym. The CCN family now comprises six identified members with properties of both positive and negative regulators of cell growth, sharing a common multimodular organization. NOV/CCN3 Protein enables heparin and binding. NOV/CCN3 Protein is involved in cell adhesion and signal transduction^[1][2][3].

Measured by its ability to mediate Balb/3T3 mouse embryonic fibroblast cell adhesion, rhNOV, immobilized at 10 µg/mL, will induce 62.85% adhesion on Balbc/3T3 cells (100 µL/well at 3 x 10⁴ cells/mL).

Canine

HEK293

C-6*His

J9P4N7 (Q29-M353)

475083  [NCBI]

QRCPTQCPARCAPTPPACAPGVRAVLDDCSCCLVCARQRGESCSPLQPCEESRGLFCDRRADPSAGGGICMAVEGDNCVFDGVIYQSGETFQPSCKYQCACRDGQIGCVPRCGEDLLLPQPDCPAPRKVEVPGECCEKWICDSNEKGELGGLALPAYRTEATLGVAVSDSGINCIEQTTEWSACSKSCGMGFSTRVTNRNPQCEMVKQTRLCMVRPCEQEHQQPADKKGKKCLRTTKSLKAIHLQFKNCTSLHTYKPRFCGVCSDGRCCTPHNTKTIQVEFQCSPGQIIKKPVMVIGTCTCHSNCPHNREAFLQELKPNTSRGEM

Approximately 42-50 due to the glycosylation

Greater than 90% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.