NR5A1 Protein, Human (His, Strep)
NR5A1 Protein, Human (His, Strep) is the recombinant human-derived NR5A1, expressed by E. coli , with Strep, His labeled tag. ,
- Species: Human
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
NR5A1 Protein, Human (His, Strep) is the recombinant human-derived NR5A1, expressed by E. coli , with Strep, His labeled tag. ,
Background
NR5A1 is a transcriptional activator essential for sexual differentiation and the formation of primary steroidogenic tissues. It binds to the Ad4 site in the promoter regions of steroidogenic P450 genes such as CYP11A, CYP11B, and CYP21B, and regulates genes like AMH/Muellerian inhibiting substance, ACH, and STAR. Its recognition consensus sequences are 5'-YCAAGGYC-3' and 5'-RRAGGTCA-3'. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter, regulating basal and cAMP-dependent transcriptional activity. NR5A1 also binds phosphatidylcholine (by similar) and phospholipids with a phosphatidylinositol (PI) headgroup, particularly PI(3,4)P2 and PI(3,4,5)P3. Phosphorylation of NR5A1 by HIPK3 activates it, enhancing steroidogenic gene expression upon cAMP signaling pathway stimulation.
Technical Parameters
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Species Human
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Source E. coli
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Tag Strep;His
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Accession
Q13285 (S218-T461, C247S, C412S)
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Gene ID2516
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Protein Length
Partial
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Synonyms
NR5A1; STF-1; Prev. FTZF1; Steroidogenic Factor 1 Nuclear Receptor; Steroidogenic Factor 1; Adrenal 4 Binding Protein; AD4BP; Adrenal 4-Binding Protein; HSF-1; Alternative Protein NR5A1; SF-1; Nuclear Receptor AdBP4; SF1; Steroidogenic Factor-1; FTZ1; SPG
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AA Sequence
SGGPNVPELILQLLQLEPDEDQVRARILGSLQEPTKSRPDQPAAFGLLCRMADQTFISIVDWARRCMVFKELEVADQMTLLQNCWSELLVFDHIYRQVQHGKEGSILLVTGQEVELTTVATQAGSLLHSLVLRAQELVLQLLALQLDRQEFVCLKFIILFSLDLKFLNNHILVKDAQEKANAALLDYTLCHYPHSGDKFQQLLLCLVEVRALSMQAKEYLYHKHLGNEMPRNNLLIEMLQAKQT
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Predicted Molecular Mass
31 kDa
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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