Nucleocapsid Protein, Virus (His, solution)
Based on 1 Customer Validation
Nucleocapsid Protein, Virus (His, solution) expressed by E. coli systems. Nucleocapsid Protein is required for coronavirus RNA synthesis and has RNA chaperone activity.
- Species: Virus
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
Nucleocapsid Protein, Virus (His, solution) expressed by E. coli systems. Nucleocapsid Protein is required for coronavirus RNA synthesis and has RNA chaperone activity[1].
Coronaviruses are enveloped viruses with a positive-sense RNA genomeand and has a nucleocapsid of helical symmetry. The Nucleocapsid Protein localize to the cytoplasm and the nucleolus in both virus-infected primary cells and in cells transfected with plasmids that express N protein[1].Nucleocapsid Protein is a basic protein and is abundant in coronaviruses, which has nonspecific binding activity toward nucleic acids, including ssRNA, ssDNA, and dsDNA. Nucleocapsid Protein is an essential RNA-binding viral protein in human coronavirus (CoV)-infected cells, is required for the replication and transcription of viral RNA. Human CoV NP is a valid target for antiviral drug research[1][2].
Immobilized 2019-nCoV NP Antibody(6G9)-Fc at 5 μg/mL(100 μl/well) can bind 2019-nCoV Nucleocapsid Protein-His. The ED50 of 2019-nCoV Nucleocapsid Protein-His is 0.1-0.7 μg/mL.
Technical Parameters
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Species Virus
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Source E. coli
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Tag N-6*His
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Accession
QHD43423.2 (M1-A419)
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Gene ID43740575 [NCBI]
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Molecular Construction
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N-term
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6*His
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Nucleocapsid (M1-A419)
Accession # QHD43423.2 -
C-term
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Protein Length
Full Length
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Synonyms
2019-nCoV coronavirus NP Protein; 2019-nCoV np Protein; 2019-nCoV novel coronavirus Nucleoprotein Protein
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AA Sequence
MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA
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Predicted Molecular Mass
49.4 kDa
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Molecular Weight
Approximately 50-60 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.22 μm filtered solution of 20 mM Tris-HCl, 300 mM NaCl, 10% glycerol, 1 mM EDTA, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (264 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Sander van Boheemen, et al. Genomic characterization of a newly discovered coronavirus associated with acute respiratory distress syndrome in humans. mBio. 2012 Nov 20;3(6):e00473-12. [Content Brief]
[2]. Chung-ke Chang, et al. Structure-based virtual screening and experimental validation of the discovery of inhibitors targeted towards the human coronavirus nucleocapsid protein. Mol Biosyst. 2016 Jan;12(1):59-66. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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