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Nucleolar transcription factor 1/UBTF Protein, Human (His-SUMO)

The nucleolar transcription factor 1/UBTF protein critically activates RNA polymerase I-mediated transcription by binding to the ribosomal RNA gene promoter and cooperating with the SL1/TIF-IB complex. Nucleolar transcription factor 1/UBTF Protein, Human (His-SUMO) is the recombinant human-derived Nucleolar transcription factor 1/UBTF protein, expressed by E. coli , with N-SUMO, N-6*His labeled tag.

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  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Nucleolar transcription factor 1/UBTF Protein, Human (His-SUMO)
  • Nucleolar transcription factor 1/UBTF Protein, Human (His-SUMO) Product Image
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[email protected] [email protected]
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

The nucleolar transcription factor 1/UBTF protein critically activates RNA polymerase I-mediated transcription by binding to the ribosomal RNA gene promoter and cooperating with the SL1/TIF-IB complex. Nucleolar transcription factor 1/UBTF Protein, Human (His-SUMO) is the recombinant human-derived Nucleolar transcription factor 1/UBTF protein, expressed by E. coli , with N-SUMO, N-6*His labeled tag.

Background

The Nucleolar transcription factor 1/UBTF protein plays a crucial role in transcriptional activation mediated by RNA polymerase I by recognizing the ribosomal RNA gene promoter and engaging in cooperative interactions with the transcription factor SL1/TIF-IB complex. Specifically binding to the upstream control element, UBTF functions as a homodimer and interacts with various proteins, including TBP, TAF1A, RASL11A, IRS1, PIK3CA, DHX33, PHF6, CEBPA (isoform 1 and isoform 4), DDX11, NOP53, and ALKBH2. These interactions highlight the protein's involvement in diverse cellular processes, emphasizing its regulatory role in ribosomal RNA gene transcription and its association with key factors implicated in transcriptional control and nucleolar function.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 90%, as determined by reducing SDS-PAGE.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag N-SUMO;N-6*His
  • Accession

    P17480 (M1-T670)

  • Gene ID
    7343  [NCBI]
  • Molecular Construction
    • N-term
    • 6*His-SUMO
    • UBTF (M1-T670)
      Accession # P17480
    • C-term
  • Protein Length

    Partial

  • Synonyms

    UBTF; Upstream Binding Transcription Factor; UBF1; UBF; Upstream Binding Transcription Factor, RNA Polymerase I; Nucleolar Transcription Factor 1; NOR-90; UBF2; Upstream-Binding Factor 1; Autoantigen NOR-90; UBF-1; Upstream Binding Transcription Factor, R

  • AA Sequence

    MNGEADCPTDLEMAAPKGQDRWSQEDMLTLLECMKNNLPSNDSSKFKTTESHMDWEKVAFKDFSGDMCKLKWVEISNEVRKFRTLTELILDAQEHVKNPYKGKKLKKHPDFPKKPLTPYFRFFMEKRAKYAKLHPEMSNLDLTKILSKKYKELPEKKKMKYIQDFQREKQEFERNLARFREDHPDLIQNAKKSDIPEKPKTPQQLWYTHEKKVYLKVRPDATTKEVKDSLGKQWSQLSDKKRLKWIHKALEQRKEYEEIMRDYIQKHPELNISEEGITKSTLTKAERQLKDKFDGRPTKPPPNSYSLYCAELMANMKDVPSTERMVLCSQQWKLLSQKEKDAYHKKCDQKKKDYEVELLRFLESLPEEEQQRVLGEEKMLNINKKQATSPASKKPAQEGGKGGSEKPKRPVSAMFIFSEEKRRQLQEERPELSESELTRLLARMWNDLSEKKKAKYKAREAALKAQSERKPGGEREERGKLPESPKRAEEIWQQSVIGDYLARFKNDRVKALKAMEMTWNNMEKKEKLMWIKKAAEDQKRYERELSEMRAPPAATNSSKKMKFQGEPKKPPMNGYQKFSQELLSNGELNHLPLKERMVEIGSRWQRISQSQKEHYKKLAEEQQKQYKVHLDLWVKSLSPQDRAAYKEYISNKRKSMTKLRGPNPKSSRTT

  • Predicted Molecular Mass

    95.2 kDa

  • Molecular Weight

    Approximately 95 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Endotoxin Level

<1.0 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 90%, as determined by reducing SDS-PAGE.

  • Nucleolar transcription factor 1/UBTF Protein, Human (His-SUMO) Product Image
    1/16
  • Experimental Validation Results for Nucleolar transcription factor 1/UBTF Protein, Human (His-SUMO)
  • Nucleolar transcription factor 1/UBTF Protein, Human (His-SUMO) Product Image