OPN1LW Protein, Human (Cell-Free, His)
OPN1LW protein, crucial for vision, is a vital element of visual pigments responsible for light absorption. The pigments consist of opsin, forming a covalent bond with cis-retinal. This molecular mechanism is indispensable for perceiving light stimuli in vision. OPN1LW Protein, Human (Cell-Free, His) is the recombinant human-derived OPN1LW protein, expressed by E. coli Cell-free , with N-10*His labeled tag.
- Species: Human
- Source: E. coli Cell-free
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
OPN1LW protein, crucial for vision, is a vital element of visual pigments responsible for light absorption. The pigments consist of opsin, forming a covalent bond with cis-retinal. This molecular mechanism is indispensable for perceiving light stimuli in vision. OPN1LW Protein, Human (Cell-Free, His) is the recombinant human-derived OPN1LW protein, expressed by E. coli Cell-free , with N-10*His labeled tag.
Background
The OPN1LW protein, integral to the process of vision, is a key component of visual pigments, which are responsible for absorbing light. These pigments comprise an apoprotein known as opsin, which forms a covalent linkage with cis-retinal, facilitating the intricate molecular mechanism essential for the perception of light stimuli in vision.
Technical Parameters
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Species Human
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Source E. coli Cell-free
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Tag N-10*His
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Accession
P04000 (M1-A364, M153L, A180S)
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Gene ID/
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Molecular Construction
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N-term
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10*His
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OPN1LW (M1-A364, M153L, A180S)
Accession # P04000 -
C-term
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Protein Length
Full Length
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Mutation
M153L, A180S
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Mutation Definition
Natural variant
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Synonyms
OPN1LW; COD5; Prev. RCP; Long-Wave-Sensitive Opsin 1; Prev. CBBM; Red-Sensitive Opsin; Prev. CBP; ROP; Opsin 1 (Cone Pigments), Long-Wave-Sensitive; Color Blindness, Protan; Red Cone Photoreceptor Pigment; Red Cone Opsin; Opsin 1, Long Wave Sensitive; Con
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AA Sequence
MAQQWSLQRLAGRHPQDSYEDSTQSSIFTYTNSNSTRGPFEGPNYHIAPRWVYHLTSVWMIFVVTASVFTNGLVLAATMKFKKLRHPLNWILVNLAVADLAETVIASTISIVNQVSGYFVLGHPMCVLEGYTVSLCGITGLWSLAIISWERWLVVCKPFGNVRFDAKLAIVGIAFSWIWSAVWTAPPIFGWSRYWPHGLKTSCGPDVFSGSSYPGVQSYMIVLMVTCCIIPLAIIMLCYLQVWLAIRAVAKQQKESESTQKAEKEVTRMVVVMIFAYCVCWGPYTFFACFAAANPGYAFHPLMAALPAYFAKSATIYNPVIYVFMNRQFRNCILQLFGKKVDDGSELSSASKTEVSSVSSVSPA
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Predicted Molecular Mass
43.4 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add 5-50% of glycerol (final concentration). Our default final concentration of glycerol is 50%. Customers could use it as reference.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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