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OSMR beta Protein, Mouse (HEK293, His)

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Based on 1 Customer Validation

The OSMR beta Protein, in partnership with IL31RA, constitutes the IL31 receptor, binding IL31 to activate STAT3, and potentially STAT1 and STAT5.OSMR beta also transduces OSM-specific signaling.It forms a heterodimer with IL6ST, creating the type II OSM receptor, and another heterodimer with IL31RA for the IL31 receptor.OSMR beta Protein, Mouse (HEK293, His) is the recombinant mouse-derived OSMR beta protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for OSMR beta Protein, Mouse (HEK293, His)
  • OSMR beta Protein, Mouse (HEK293, His) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

The OSMR beta Protein, in partnership with IL31RA, constitutes the IL31 receptor, binding IL31 to activate STAT3, and potentially STAT1 and STAT5.OSMR beta also transduces OSM-specific signaling.It forms a heterodimer with IL6ST, creating the type II OSM receptor, and another heterodimer with IL31RA for the IL31 receptor.OSMR beta Protein, Mouse (HEK293, His) is the recombinant mouse-derived OSMR beta protein, expressed by HEK293 , with C-His labeled tag.

Background

The OSMR beta Protein associates with IL31RA to form the IL31 receptor. It binds IL31, triggering the activation of STAT3, and possibly STAT1 and STAT5 (By similarity). Additionally, OSMR beta is capable of transducing OSM-specific signaling events (By similarity). It forms a heterodimer with IL6ST, constituting the type II OSM receptor, and another heterodimer with IL31RA to shape the IL31 receptor.

Verified Bioactivity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse OSM R beta is immobilized at 0.5 µg/mL and Recombinant Mouse gp130 at 0.5 µg/mL (100 µL/well) can bind Recombinant Mouse OSM. The ED50 for this effect is 0.9183 ng/mL.

MCE Validation Data

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. When Recombinant Mouse OSM R beta is immobilized at 0.5 µg/mL and Recombinant Mouse gp130 at 0.5 µg/mL (100 µL/well) can bind Recombinant Mouse OSM. The ED50 for this effect is 0.9183 ng/mL.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-8*His
  • Accession

    O70458 (E24-M737)

  • Gene ID
    18414  [NCBI]
  • Molecular Construction
    • N-term
    • OSMR beta (E24-M737)
      Accession # O70458
    • 8*His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    OSMR; IL-31 Receptor Subunit Beta; Oncostatin M Receptor; IL-31R Subunit Beta; OSMRB; IL-31R-Beta; Oncostatin-M-Specific Receptor Subunit Beta; IL-31RB; OSMRbeta; Oncostatin-M Specific Receptor Beta Subunit; Interleukin-31 Receptor Subunit Beta; PLCA1

  • AA Sequence

    EVLEEPLPLTPEIHKVSFQLKLQEVNLEWTVPALTHEELNMIFQIEISRLNISNTIWVENYSTTVKREEAVRWNWTSDIPLECVKHFIRIRALVDDTKSLPQSSWGNWSSWKEVNAKVSVEPDKSLIFPKDKVLEEGSNVTICLMYGQNVYNVSCKLQDEPIHGEQLDSHVSLLKLNNVVFLSDTGTNINCQATKGPKRIFGTVLFVSKVLEEPKNVSCETRDFKTLDCSWEPGVDTTLTWRKQRFQNYTLCESFSKRCEVSNYRNSYTWQITEGSQEMYNFTLTAENQLRKRSVNINFNLTHRVHPKAPQDVTLKIIGATKANMTWKVHSHGNNYTLLCQVKLQYGEVIHEHNVSVHMSANYLFSDLDPDTKYKAFVRCASANHFWKWSDWTQKEFSTPETAPSQALDVWRQVWSENGRRIVTLFWKPLLKSQANGKIISYNIVVENEAKPTESEHYCVWAPALSTNLSLDLQPYKIRITTNNSMGASPESLMVLSNDSGHEEVKEKTIKGIKDAFNISWEPVSGDTMGYVVDWCAHSQDQRCDLQWKNLGPNTTSTTITSDDFKPGVRYNFRIFERSVEHKARLVEKQRGYTQELAPLVNPKVEIPYSTPNSFVLRWPDYDSDFQAGFIKGYLVYVKSKEMQCNQPWERTLLPDNSVLCKYDINGSETKTLTVENLQPESLYEFFVTPYTSAGPGPNETFTKVTTPDARSHM

  • Predicted Molecular Mass

    83.2 kDa

  • Molecular Weight

    Approximately 115-140 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by Bis-Tris PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. When Recombinant Mouse OSM R beta is immobilized at 0.5 µg/mL and Recombinant Mouse gp130 at 0.5 µg/mL (100 µL/well) can bind Recombinant Mouse OSM. The ED50 for this effect is 0.9183 ng/mL.

  • OSMR beta Protein, Mouse (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for OSMR beta Protein, Mouse (HEK293, His)
  • OSMR beta Protein, Mouse (HEK293, His) Product Image