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OV16 Protein, Onchocerca volvulus (GST)

Cat. No.: HY-P78691
COA Handling Instructions

The OV16 protein is a phosphatidylethanolamine-binding protein found in the subcutaneous tissue, stratum corneum, and uterus and has been shown to be involved in multiple physiological functions, possibly related to structural integrity and reproduction. It belongs to the phosphatidylethanolamine-binding protein family, suggesting a role in lipid processes or signaling. OV16 Protein, Onchocerca volvulus (GST) is the recombinant OV16 protein, expressed by HEK293 , with N-GST labeled tag. The total length of OV16 Protein, Onchocerca volvulus (GST) is 181 a.a., with molecular weight of ~45 kDa.

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Description

The OV16 protein is a phosphatidylethanolamine-binding protein found in the subcutaneous tissue, stratum corneum, and uterus and has been shown to be involved in multiple physiological functions, possibly related to structural integrity and reproduction. It belongs to the phosphatidylethanolamine-binding protein family, suggesting a role in lipid processes or signaling. OV16 Protein, Onchocerca volvulus (GST) is the recombinant OV16 protein, expressed by HEK293 , with N-GST labeled tag. The total length of OV16 Protein, Onchocerca volvulus (GST) is 181 a.a., with molecular weight of ~45 kDa.

Background

OV16 Protein, a member of the phosphatidylethanolamine-binding protein family, is localized in the hypodermis, cuticle, and uterus. This protein's distribution across these cellular structures suggests its involvement in diverse physiological functions, potentially related to structural integrity and reproductive processes. The designation within the phosphatidylethanolamine-binding protein family hints at its putative role in lipid-related processes or cellular signaling. The specific localization in the hypodermis and cuticle may imply functions related to structural support or protection, while its presence in the uterus suggests a potential role in reproductive biology or embryonic development. The broader implications of OV16 Protein within these cellular contexts underscore its significance in various aspects of cellular and organismal biology.

Biological Activity

Data is not available.

Species

Others

Source

HEK293

Tag

N-GST

Accession

P31729-1 (K17-D197)

Gene ID

/

Molecular Construction
N-term
GST
OV16 (K17-D197)
Accession # P31729-1
C-term
Synonyms
OV16; OV-16 antigen
AA Sequence

KISAENANCKKCTPMLVDSAFKEHGIVPDVVSTAPTKLVNVSYNNLTVNLGNELTPTQVKNQPTKVSWDAEPGALYTLVMTDPDAPSRKNPVFREWHHWLIINISGQNVSSGTVLSDYIGSGPRKGTGLHRYVFLVYKQPGSITDTQHGGNRRNFKVMDFANKHHLGNPVAGNFFQAKHED

Molecular Weight

Approximately 45 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder.

Formulation

Lyophilized a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

OV16 Protein, Onchocerca volvulus (GST) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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OV16 Protein, Onchocerca volvulus (GST)
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HY-P78691
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